Accurate in vivo real-time determination of the hydrogen concentration in different tissues of mice after hydrogen inhalation.
Wenjun Zhu, Qianqian Gu, Boyan Liu, Yanhong Si, Huirong Sun, Jingjie Zhong, Yi Lu, Dan Wang, Junli Xue, Shucun Qin · Heliyon · 2022
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In mice, attentively, the difference of H₂ saturation concentration detected in the same tissue was directly proportional to the difference of inhaled exogenous H₂ concentration, namely, the saturation values after inhaling 42% and 67% H₂ were about 12.3- and 17.8-time greater than that after 4% H₂ inhalation in various tissues except for the gastrocnemius with 67% (about 27.1-time). These preclinical findings add evidence about the molecular-hydrogen effects, outcomes, and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas affected the outcomes measured in mice. Attentively, the difference of H₂ saturation concentration detected in the same tissue was directly proportional to the difference of inhaled exogenous H₂ concentration, namely, the saturation values after inhaling 42% and 67% H₂ were about 12.3- and 17.8-time greater than that after 4% H₂ inhalation in various tissues except for the gastrocnemius with 67% (about 27.1-time).
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 67% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 67% source-reported H₂ gas concentration. Attentively, the difference of H₂ saturation concentration detected in the same tissue was directly proportional to the difference of inhaled exogenous H₂ concentration, namely, the saturation values after inhaling 42% and 67% H₂ were about 12.3- and 17.8-time greater than that after 4% H₂ inhalation in various tissues except for the gastrocnemius with 67% (about 27.1-time). The authors concluded that different letters indicate significant differences of the saturation concentration of H₂ in the same tissue between the mice and rats ( P < 0.05).
Why These Findings Matter
This publication adds useful evidence about where molecular hydrogen did and did not change the measured outcomes, helping define the larger therapeutic evidence base.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The study used a preclinical animal experiment. The hydrogen delivery method was inhaled hydrogen gas. The source-reported hydrogen concentration was 67% source-reported H₂ gas concentration.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.