Hydrogen Promotes the M1 Macrophage Conversion During the Polarization of Macrophages in Necrotizing Enterocolitis.
Shenghua Yu, ZhiBao Lv, Zhimei Gao, Jingyi Shi, Qingfeng Sheng, Lulu Zheng, Junmei Zhou, Xueli Wang · Frontiers in pediatrics · 2021
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Independent study record
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H2HUBB TAKEAWAY
In For immunophenotypic analysis, macrophages were gently detached with cold phosphate-buffered saline (PBS), pipetted into single cells, and then suspended at 1 × 10 6 cells/ml, the NF-κB p65 was decreased in M1 macrophage cell nucleus after treatment with hydrogen compared to M1 macrophages ( t = 16.65, p < 0.05) and significantly increased in M1 macrophages after being induced by LPS and IFN-γ. The authors concluded that hydrogen could attenuate the severity of NEC through promoting M1 macrophages conversion by inhibited the expression of NF-κB p65 in the nucleus. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how hydrogen-rich water affected the outcomes measured in For immunophenotypic analysis, macrophages were gently detached with cold phosphate-buffered saline (PBS), pipetted into single cells, and then suspended at 1 × 10 6 cells/ml. The NF-κB p65 was decreased in M1 macrophage cell nucleus after treatment with hydrogen compared to M1 macrophages ( t = 16.65, p < 0.05) and significantly increased in M1 macrophages after being induced by LPS and IFN-γ.
What the Researchers Studied
The researchers studied For immunophenotypic analysis, macrophages were gently detached with cold phosphate-buffered saline (PBS), pipetted into single cells, and then suspended at 1 × 10 6 cells/ml. The study used a in vitro cell-culture laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
The preparation of hydrogen-rich water was performed as described previously ( 18 ). Hydrogen was dissolved in water or DMEM medium for 4 h under high pressure (0.4 MPa) up to a supersaturation. The saturated hydrogen water or DMEM medium was sterilized by gamma radiation and stored under atmospheric pressure at 4°C. The NF-κB p65 was decreased in M1 macrophage cell nucleus after treatment with hydrogen compared to M1 macrophages ( t = 16.65, p < 0.05) and significantly increased in M1 macrophages after being induced by LPS and IFN-γ. The authors concluded that hydrogen could attenuate the severity of NEC through promoting M1 macrophages conversion by inhibited the expression of NF-κB p65 in the nucleus. Pro-inflammatory factors and CD16/32 in M1 macrophages were decreased, and the expression of CD16/32 in lamina propria were inhibited after treatment with hydrogen, but the changes has no effects in other tissues.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was For immunophenotypic analysis, macrophages were gently detached with cold phosphate-buffered saline (PBS), pipetted into single cells, and then suspended at 1 × 10 6 cells/ml. The study used a in vitro cell-culture laboratory experiment. The hydrogen delivery method was hydrogen-rich water.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.