An LCMS-based untargeted metabolomics protocol for cochlear perilymph: highlighting metabolic effects of hydrogen gas on the inner ear of noise exposed Guinea pigs.
Kristian Pirttilä, Pernilla Videhult Pierre, Jakob Haglöf, Mikael Engskog, Mikael Hedeland, Göran Laurell, Torbjörn Arvidsson, Curt Pettersson · Metabolomics : Official journal of the Metabolomic Society · 2019
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Independent study record
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H2HUBB TAKEAWAY
The researchers found a clear difference in the perilymph metabolome of noise exposed guinea pigs with and without H₂ treatment. These results are preclinical and suggest molecular hydrogen's therapeutic potential in the condition studied. Further human research is needed to establish clinical effectiveness.
What the Researchers Studied
The study examined to present a protocol for untargeted metabolomics of guinea pig perilymph and investigate the effect of H₂ administration on the perilymph metabolome of noise exposed guinea pigs.
How Molecular Hydrogen Was Used
The left ear of guinea pigs were exposed to hazardous impulse noise only (Noise, n = 10), noise and H₂ (Noise + H₂, n = 10), only H₂ (H₂, n = 4), or untreated (Control, n = 2).
What the Researchers Found
MVDA allowed separation of groups Noise and Noise + H₂ in both the exposed and unexposed ear and yielded 15 metabolites with differentiating relative abundances.
H₂ Mechanisms / Biological Findings
Hydrogen gas (H₂) can alleviate the damage caused by oxidative stress and can be easily administered through inhalation.
Authors’ Conclusion
The authors concluded that a HILIC-UHPLC-Q-TOF-MS-based protocol for untargeted metabolomics of perilymph is presented and shown to be fit-for-purpose. The researchers found a clear difference in the perilymph metabolome of noise exposed guinea pigs with and without H₂ treatment.