Antioxidant Properties of Hydrogen Gas Attenuates Oxidative Stress in Airway Epithelial Cells.
In-Soo You, Subham Sharma, Ailyn Fadriquela, Johny Bajgai, Thuy Trinh Thi, Md Habibur Rahman, Jaeyong Sung, Hwang-Un Kwon, So-Yeon Lee, Cheol-Su Kim, Kyu-Jae Lee · Molecules (Basel, Switzerland) · 2021
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In A549 and NCI-H292 cells utilized in this study were obtained from the Korean Cell Line Bank (Cancer Research Institute of Seoul National University, Seoul, Korea), H₂O₂ stimulation increased intracellular Ca 2+ concentrations in A549 cells compared with controls ( p < 0.001). But this effect was significantly reduced in a time-dependent manner after treatment with H₂ gas ( Figure 5 A). The authors concluded that this study suggests that H₂ gas can rescue airway epithelial cells from H₂O₂ and LPS-induced oxidative stress and may be a potential intervention for airway diseases. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in A549 and NCI-H292 cells utilized in this study were obtained from the Korean Cell Line Bank (Cancer Research Institute of Seoul National University, Seoul, Korea). H₂O₂ stimulation increased intracellular Ca 2+ concentrations in A549 cells compared with controls ( p < 0.001), but this effect was significantly reduced in a time-dependent manner after treatment with H₂ gas ( Figure 5 A).
What the Researchers Studied
The researchers studied A549 and NCI-H292 cells utilized in this study were obtained from the Korean Cell Line Bank (Cancer Research Institute of Seoul National University, Seoul, Korea). The study used a in vitro cell-culture laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Reported treatment duration: 30 min. H₂O₂ stimulation increased intracellular Ca 2+ concentrations in A549 cells compared with controls ( p < 0.001), but this effect was significantly reduced in a time-dependent manner after treatment with H₂ gas ( Figure 5 A). The authors concluded that this study suggests that H₂ gas can rescue airway epithelial cells from H₂O₂ and LPS-induced oxidative stress and may be a potential intervention for airway diseases. This study investigated the role of H₂ gas in oxidative stress-induced alveolar and bronchial airway injury, where A549 and NCI-H292 cells were stimulated with hydrogen peroxide (H₂O₂) and lipopolysaccharide (LPS) in vitro.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was A549 and NCI-H292 cells utilized in this study were obtained from the Korean Cell Line Bank (Cancer Research Institute of Seoul National University, Seoul, Korea). The study used a in vitro cell-culture laboratory experiment. The reported treatment duration was 30 min.
Limitations and Safety
Safety information: In addition to this, the safety of using 2% H₂ has was also proved through the work of Ohta et al. [ 24, 25, 26 ].
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.