Controlled release of hydrogen by implantation of magnesium induces P53-mediated tumor cells apoptosis.
Rui Zan, Hao Wang, Weijie Cai, Jiahua Ni, Bérengère J C Luthringer-Feyerabend, Wenhui Wang, Hongzhou Peng, Weiping Ji, Jun Yan, Jiazeng Xia, Yang Song, Xiaonong Zhang · Bioactive materials · 2022
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In a laboratory model, when the exposed surface area of Mg was increased from 17.3 mm 2 to 51.9 mm 2, the estimated local concentration of hydrogen from 91.2 μL/d/mm 3 to 273.6 μL/d/mm 3; accordingly, the volume of tumors at 24 d declined by 64%. The authors concluded that 5 In conclusion, the researchers showed an H₂ concentration-dependent suppression of tumor cells HCT116 induced by the degradation of Mg. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas was evaluated in this publication and summarizes the source-grounded findings below. When the exposed surface area of Mg was increased from 17.3 mm 2 to 51.9 mm 2, the estimated local concentration of hydrogen from 91.2 μL/d/mm 3 to 273.6 μL/d/mm 3; accordingly, the volume of tumors at 24 d declined by 64%.
What the Researchers Studied
The study used a in vitro cell-culture laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
2.3 HP-Mg sheets were immersed in 10 mL PBS solution with different pH values (pH = 7.4, 6.5, 5.6), and the released H₂ was collected through an inverted funnel and its total volume was numerated through a calibrated burette, which was described in detail in previous work [ 28 ]. The viability of cells after exposed to hydrogen at different concentrations was tested by a Cell Counting Kit-8 assay (DOJINDO, Japan) at time points of t = 24, 48, and 72 h. 2.7 After exposed to H₂ for 12 h, tumor cells were transferred and seeded in the cell culture slide. When the exposed surface area of Mg was increased from 17.3 mm 2 to 51.9 mm 2, the estimated local concentration of hydrogen from 91.2 μL/d/mm 3 to 273.6 μL/d/mm 3; accordingly, the volume of tumors at 24 d declined by 64%. The authors concluded that 5 In conclusion, the researchers showed an H₂ concentration-dependent suppression of tumor cells HCT116 induced by the degradation of Mg. However, the underlying mechanism of hydrogen's role in cancer treatment remains ambiguous.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The study used a in vitro cell-culture laboratory experiment. The hydrogen delivery method was inhaled hydrogen gas.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.