Hydrogen gas inhalation improves delayed brain injury by alleviating early brain injury after experimental subarachnoid hemorrhage.
Kosuke Kumagai, Terushige Toyooka, Satoru Takeuchi, Naoki Otani, Kojiro Wada, Arata Tomiyama, Kentaro Mori · Scientific reports · 2020
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
Time course of ICP showed no significant difference between the control (n = 35) and H₂ (n = 33) groups (at 0, peak, 10, 20 and 30 min; P > 0.05, respectively). This source-grounded publication contributes evidence relevant to molecular hydrogen and is retained in a transparent general research category when a more specific study design is not supported by the indexed record.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas was evaluated in this publication and summarizes the source-grounded findings below. Time course of ICP showed no significant difference between the control (n = 35) and H₂ (n = 33) groups (at 0, peak, 10, 20 and 30 min; P > 0.05, respectively).
What the Researchers Studied
The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 1.3% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 1.3% source-reported H₂ gas concentration; reported treatment duration: 30 min. Time course of ICP showed no significant difference between the control (n = 35) and H₂ (n = 33) groups (at 0, peak, 10, 20 and 30 min; P > 0.05, respectively). The authors concluded that the potent antioxidant effect of H₂ is mediated by selective inhibition of highly toxic ROS such as hydroxyl radical (OH·) and peroxynitrite (ONOO − ) 6. Immunoblot analysis of the obtained brain tissue lysates was performed as described previously 17 using the following primary antibodies: mouse monoclonal anti-glial fibrillary acidic protein (GFAP) (Cell Signaling Technology, #3670; 1:1,000); rabbit monoclonal anti-S100B (GeneTex, #GTX129573; 1:1,000); rabbit monoclonal anti-phosphorylated JNK (p-JNK) (Cell Signaling Technology, #4668; 1:1,000); and rabbit monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (Cell Signaling Technology, #8884; 1:1,000).
Why These Findings Matter
This publication adds useful evidence about where molecular hydrogen did and did not change the measured outcomes, helping define the larger therapeutic evidence base.
How Strong Is This Evidence?
H2HUBB classifies this publication as other research and interprets only the outcomes directly supported by the source record.
Technical Study Details
H2HUBB classifies this publication as other research with other supported evidence. The reported sample size was 101. The hydrogen delivery method was inhaled hydrogen gas. The source-reported hydrogen concentration was 1.3% source-reported H₂ gas concentration. The reported treatment duration was 30 min.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.