Hydrogen gas inhalation protects against cutaneous ischaemia/reperfusion injury in a mouse model of pressure ulcer.
Wei Fang, Guizhen Wang, Luyan Tang, Huilin Su, Huyan Chen, Wanqing Liao, Jinhua Xu · Journal of cellular and molecular medicine · 2018
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Independent study record
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H2HUBB TAKEAWAY
In mice, the ulcer size in control mice at 4 days after reperfusion was assigned a value of 100%. * P <.05; ** P <.01; *** P <.001 compared to control 3.2 Haematoxylin and eosin (HE) staining showed that H₂ inhalation reduced inflammatory cell infiltration and tissue necrosis in skin wound caused by I/R cycles (Figure S1 ). These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas affected the outcomes measured in mice. The ulcer size in control mice at 4 days after reperfusion was assigned a value of 100%. * P <.05; ** P <.01; *** P <.001 compared to control 3.2 Haematoxylin and eosin (HE) staining showed that H₂ inhalation reduced inflammatory cell infiltration and tissue necrosis in skin wound caused by I/R cycles (Figure S1 ).
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 75% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 75% source-reported H₂ gas concentration; reported treatment duration: 24 hours. The ulcer size in control mice at 4 days after reperfusion was assigned a value of 100%. * P <.05; ** P <.01; *** P <.001 compared to control 3.2 Haematoxylin and eosin (HE) staining showed that H₂ inhalation reduced inflammatory cell infiltration and tissue necrosis in skin wound caused by I/R cycles (Figure S1 ). The authors concluded that taken together, these data indicated that H₂ inhalation suppressed the formation of pressure ulcer in a mouse model. The therapeutic effects of molecular hydrogen might be related to its antioxidant, anti-inflammatory, pro-healing actions. Figure 4 Hydrogen activated the NRF 2‐ ARE pathway in wounded tissue.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The reported sample size was 1. The study used a preclinical animal experiment. The hydrogen delivery method was inhaled hydrogen gas. The source-reported hydrogen concentration was 75% source-reported H₂ gas concentration. The reported treatment duration was 24 hours.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.