Hydrogen Research Study
← Back to Research Library
Laboratory or cellular evidenceLaboratory StudyOther or not reported

Hydrogen gas protects IP3Rs by reducing disulfide bridges in human keratinocytes under oxidative stress.

Ching-Ying Wu, Wen-Li Hsu, Ming-Hsien Tsai, Jui-Lin Liang, Jian-He Lu, Chia-Jung Yen, Hsin-Su Yu, Mami Noda, Chi-Yu Lu, Chu-Huang Chen, Shian-Jang Yan, Tohru Yoshioka · Scientific reports · 2017

Research-use notice

Independent study record

Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.

H2HUBB Research Library branded molecular hydrogen research image
Primary topic Molecular Hydrogen Overview
Evidence type Laboratory or cellular evidence
Publication type Laboratory Study
Hydrogen method Other or not reported

H2HUBB TAKEAWAY

In Decreased Ca2+ signaling is observed in aged cells, probably secondary to the formation of disulfide bonds among Ca2+ signaling-related proteins, hydrogen peroxide (H₂O₂) elicits ROS that induces skin aging through oxidation of proteins, forming disulfide bridges with cysteine or methionine sulfhydryl groups. The authors concluded that in conclusion, skin aging processes may involve ROS-induced protein dysfunction due to disulfide bond formation, and H₂ can protect oxidation of this process. These findings come from a laboratory model and suggest molecular hydrogen's biological potential. Further research is needed to determine clinical relevance.

What the Researchers Tested

In vitro cell-culture laboratory experiment.

Laboratory Model

Decreased Ca2+ signaling is observed in aged cells, probably secondary to the formation of disulfide bonds among Ca2+ signaling-related proteins.

How Molecular Hydrogen Was Used

Hydrogen peroxide (H₂O₂) elicits ROS that induces skin aging through oxidation of proteins, forming disulfide bridges with cysteine or methionine sulfhydryl groups. Molecular hydrogen (H₂) was found to be more effectively protected H₂O₂-induced IP3R1 dysfunction by reducing disulfide bonds, rather than quenching ROS. In conclusion, skin aging processes may involve ROS-induced protein dysfunction due to disulfide bond formation, and H₂ can protect oxidation of this process.

What Molecular Hydrogen Changed

Hydrogen peroxide (H₂O₂) elicits ROS that induces skin aging through oxidation of proteins, forming disulfide bridges with cysteine or methionine sulfhydryl groups.

Authors’ Conclusion

These findings come from a laboratory model and suggest molecular hydrogen's biological potential. Further research is needed to determine clinical relevance.