Hydrogen inhalation ameliorated mast cell-mediated brain injury after intracerebral hemorrhage in mice.
Anatol Manaenko, Tim Lekic, Qingyi Ma, John H Zhang, Jiping Tang · Critical care medicine · 2013
Research-use notice
Independent study record
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H2HUBB TAKEAWAY
In a laboratory model, hydrogen treatment diminished phosphorylation of Lyn kinase and release of tryptase, decreased accumulation and degranulation of mast cells, attenuated blood-brain barrier disruption, and improved neurobehavioral function. The authors concluded that hydrogen inhalation preserved blood-brain barrier disruption by prevention of mast cell activation after intracerebral hemorrhage. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen was evaluated in this publication and summarizes the source-grounded findings below. Hydrogen treatment diminished phosphorylation of Lyn kinase and release of tryptase, decreased accumulation and degranulation of mast cells, attenuated blood-brain barrier disruption, and improved neurobehavioral function.
What the Researchers Studied
The study used a laboratory experiment.
What Effects Did Molecular Hydrogen Have?
One hour after operation, animals were placed in chamber connected to a hydrogen gas tank. The hydrogen treatment chamber was continually flushed with gas mix 2.9% hydrogen and 21% oxygen balanced with nitrogen, flow rate 8 l/minute. Hydrogen concentration was controlled at outlet using a Hydrogen-meter (H₂ Scan, Valencia, CA, USA). Hydrogen treatment diminished phosphorylation of Lyn kinase and release of tryptase, decreased accumulation and degranulation of mast cells, attenuated blood-brain barrier disruption, and improved neurobehavioral function. The authors concluded that hydrogen inhalation preserved blood-brain barrier disruption by prevention of mast cell activation after intracerebral hemorrhage. Those studies demonstrated that hydrogen therapy reduced ROS levels and postulated that its beneficial effects are linked to its anti-oxidative properties. However, pathways leading to diminished oxidative stress following hydrogen treatment remain to be elucidated.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The reported sample size was 171. The study used a laboratory experiment.
Limitations and Safety
Reported limitations: One limitation of the modelis the inherent inflammatory reaction caused by collagenase.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.