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Human clinical evidenceHuman Clinical Studyinhaled hydrogen gas

Hydrogen gas inhalation prior to high-intensity training reduces attenuation of nitric oxide bioavailability in male rugby players.

Yuqi Zhao, Chaoqun Li, Shi Zhou, Zhiguang Xu, Xin Huang, Li Wen · PeerJ · 2024

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Independent study record

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H2HUBB Research Library branded molecular hydrogen research image
Primary topic Exercise, Athletics, and Recovery
Evidence type Human clinical evidence
Publication type Human Clinical Study
Hydrogen method inhaled hydrogen gas

H2HUBB TAKEAWAY

Hydrogen inhalation preserved nitric-oxide bioavailability markers after high-intensity training in male rugby players compared with placebo.

What the Researchers Studied

The aims of this study were to determine the effects of H₂ inhalation on plasma nitric oxide (NO) level and its synthesis precursor in professional athletes.

How Molecular Hydrogen Was Used

Molecular hydrogen was administered as participants underwent 1 week of h₂ supplementation and 1 week of placebo treatment prior to daily sessions of high-intensity exercise training, separated by 1 week of low-intensity training as a washout.

What the Researchers Found

Two-way (supplementation and time) repeated-measures analyses of variance showed that NO, L-arginine, and tetrahydrobiopterin levels in the H₂ inhalation group were significantly higher than those in the placebo group after exercise (D6) and remained higher after 24 h of rest (D7). Levels of hydroxydeoxyguanosine and interleukin 6 were lower in the H₂ inhalation week than in the placebo week on D6 and D7. Total antioxidant levels were significantly higher with H₂ inhalation than with placebo.

H₂ Mechanisms / Biological Findings

Inhalation of hydrogen gas (H₂) as an antioxidant supplement may alleviate exercise-induced oxidative damage and protect post-exercise hydrogen peroxide signaling, which may help mediate beneficial exercise adaptation.

Authors’ Conclusion

The authors concluded that these results suggest that H₂ inhalation helps to maintain NO signaling after exercise and to alleviate inflammation and oxidative stress induced by high-intensity exercise training in professional athletes.