Therapeutic efficacy of hydrogen‑rich saline alone and in combination with PI3K inhibitor in non‑small cell lung cancer.
Yu Jiang, Gang Liu, Li Zhang, Sheng Cheng, Chun Luo, Yang Liao, Shuliang Guo · Molecular medicine reports · 2018
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Independent study record
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H2HUBB TAKEAWAY
In The human NSCLC A549 cell line was obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China), to investigate the proliferation inhibition effects of hydrogen-rich saline alone and in combination with LY-294002 in A549 cells, the cells were treated with hydrogen-rich saline, LY-294002 or a combination of the two for 48 h. The inhibition of cell proliferation rate was measured using an MTT assay. The authors concluded that the results further suggested that the combination therapy may reduce cell proliferation and promote cellular apoptosis by downregulating Akt phosphorylation and inhibiting the PI3K pathway in NSCLC cell lines. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in The human NSCLC A549 cell line was obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). To investigate the proliferation inhibition effects of hydrogen-rich saline alone and in combination with LY-294002 in A549 cells, the cells were treated with hydrogen-rich saline, LY-294002 or a combination of the two for 48 h, and the inhibition of cell proliferation rate was measured using an MTT assay.
What the Researchers Studied
The researchers studied The human NSCLC A549 cell line was obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The study used a in vitro cell-culture laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
A549 cells were grown in 96-well plates at a density of 5×10 3 cells per well for 24 h, prior to being treated with different concentrations of LY294002 (primary concentration, 20 µM, the concentration of each well is 1/3 of the previous well), hydrogen-rich saline (with a hydrogen concentration of 0.6 mmol/l) or a combination of hydrogen-rich saline and LY294002. To investigate the proliferation inhibition effects of hydrogen-rich saline alone and in combination with LY-294002 in A549 cells, the cells were treated with hydrogen-rich saline, LY-294002 or a combination of the two for 48 h, and the inhibition of cell proliferation rate was measured using an MTT assay. The authors concluded that the results further suggested that the combination therapy may reduce cell proliferation and promote cellular apoptosis by downregulating Akt phosphorylation and inhibiting the PI3K pathway in NSCLC cell lines. The aim of the present study was to investigate the effects of combination therapy of LY294002, a specific inhibitor of phosphatidylinositol 3‑kinase (PI3K), with hydrogen‑rich saline on the proliferation and apoptosis of the non‑small cell lung cancer (NSCLC) A549 cell line and the mechanisms underpinning this. The present study investigated the effect of hydrogen‑rich saline alone and in combination with the PI3K inhibitor, LY294002, on the proliferation, oxidative stress and apoptosis of NSCLC A549 cells.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was The human NSCLC A549 cell line was obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). The study used a in vitro cell-culture laboratory experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.