Hydrogen Research Study
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Preclinical animal evidenceAnimal studyOther or not reported

Hydrogen-rich saline injection into the subarachnoid cavity within 2 weeks promotes recovery after acute spinal cord injury.

Jian-Long Wang, Qing-Shan Zhang, Kai-di Zhu, Jian-Feng Sun, Ze-Peng Zhang, Jian-Wen Sun, Ke-Xiang Zhang · Neural regeneration research · 2015

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Independent study record

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H2HUBB Research Library branded molecular hydrogen research image
Primary topic Neurological Conditions
Evidence type Preclinical animal evidence
Publication type Animal study
Hydrogen method Other or not reported

H2HUBB TAKEAWAY

In rats, at 24 hours, 48 hours, 1 week and 2 weeks after injury showed that hydrogen-rich saline markedly reduced cell death, inflammatory cell infiltration, serum malondialdehyde content, and caspase-3 immunoreactivity, elevated serum superoxide dismutase activity and calcitonin gene-related peptide immunoreactivity, and improved motor function in the hindlimb. These results are preclinical and suggest molecular hydrogen's therapeutic potential in the condition studied. Further human research is needed to establish clinical effectiveness.

What the Researchers Studied

The study examined rats.

How Molecular Hydrogen Was Used

Injection of hydrogen-rich saline is an effective method for transporting molecular hydrogen. At 0.5, 1, 2, 4, 8, 12 and 24 hours after injury, then once daily for 2 weeks, 0.25 mL/kg hydrogen-rich saline was infused into the subarachnoid space through a catheter. The present study confirms that hydrogen-rich saline injected within 2 weeks of injury effectively contributes to the repair of spinal cord injury in the acute stage.

What the Researchers Found

At 24 hours, 48 hours, 1 week and 2 weeks after injury showed that hydrogen-rich saline markedly reduced cell death, inflammatory cell infiltration, serum malondialdehyde content, and caspase-3 immunoreactivity, elevated serum superoxide dismutase activity and calcitonin gene-related peptide immunoreactivity, and improved motor function in the hindlimb.

H₂ Mechanisms / Biological Findings

At 24 hours, 48 hours, 1 week and 2 weeks after injury showed that hydrogen-rich saline markedly reduced cell death, inflammatory cell infiltration, serum malondialdehyde content, and caspase-3 immunoreactivity, elevated serum superoxide dismutase activity and calcitonin gene-related peptide immunoreactivity, and improved motor function in the hindlimb. Hydrogen can relieve tissue-damaging oxidative stress, inflammation and apoptosis.

Authors’ Conclusion

A distinct authors’ conclusion was not available in the source material reviewed by H2HUBB.