In vitro fermentation properties of magnesium hydride and related modulation effects on broiler cecal microbiome and metabolome.
Heng Hu, He Zhu, Haiyan Yang, Wen Yao, Weijiang Zheng · Frontiers in microbiology · 2023
Research-use notice
Independent study record
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H2HUBB TAKEAWAY
In In vitro fermentation models provide a fast, reproducible, and direct assessment tool for microbiota metabolism and composition, this aligns with previous findings, where hydrogen-rich water treatment for 20 days improved the relative abundance of Firmicutes and decreased the relative abundance of Proteobacteria in mice feces ( Lian et al., 2021 ). The authors concluded that in summary, the results of this study suggested that both MGH and coated-MGH have similar yet distinct positive effects on the microbiota and metabolites of the broiler cecal in an in vitro fermentation model. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how hydrogen-rich water affected the outcomes measured in In vitro fermentation models provide a fast, reproducible, and direct assessment tool for microbiota metabolism and composition. This aligns with previous findings, where hydrogen-rich water treatment for 20 days improved the relative abundance of Firmicutes and decreased the relative abundance of Proteobacteria in mice feces ( Lian et al., 2021 ).
What the Researchers Studied
The researchers studied In vitro fermentation models provide a fast, reproducible, and direct assessment tool for microbiota metabolism and composition. The study used a in vitro cell-culture laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
SCFAs concentrations in the fermentation liquids were analyzed by gas chromatography (GC-14A with an FID detector; Shimadzu, Japan; capillary column: 30 m × 0.32 mm × 0.25 μm film thickness) with a H₂ flame ionization detector and split injection as previously described ( Mao et al., 2007 ). To measure the hydrogen production in the collected has samples at different timepoints, a combination of a gas-tight plastic chamber (0.325 m*0.225 m*0.155 m) and a hydrogen gas detector (Ennix GS40 Ennix Gmbh, Eichingen, Germany) was utilized. The hydrogen volume was calculated using the following formula: Hydrogen detector reading (ppm)* Volume of the gas-tight plastic chamber (m3) = Volume of hydrogen in the gas-tight plastic chamber (mL). This aligns with previous findings, where hydrogen-rich water treatment for 20 days improved the relative abundance of Firmicutes and decreased the relative abundance of Proteobacteria in mice feces ( Lian et al., 2021 ). The authors concluded that in summary, the results of this study suggested that both MGH and coated-MGH have similar yet distinct positive effects on the microbiota and metabolites of the broiler cecal in an in vitro fermentation model. Blautia is involved in the non-functional Wood-Ljungdahl pathway (WLP) of H₂ + CO₂ acetylation, where hydrogen serves as an electron donor to reduce carbon dioxide to acetic acid ( Trischler et al., 2022 ).
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was In vitro fermentation models provide a fast, reproducible, and direct assessment tool for microbiota metabolism and composition. The reported sample size was 5. The study used a in vitro cell-culture laboratory experiment. The hydrogen delivery method was hydrogen-rich water.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.