Influence of hydrogen-occluding-silica on migration and apoptosis in human esophageal cells in vitro.
Qiang Li, Yoshiharu Tanaka, Nobuhiko Miwa · Medical gas research · 2017
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In These findings should provide insights into the mechanism of inhibition of H₂-silica on human cancer cells in vitro, apoptosis-inducing effect on KYSE-70 cells was observed in 10, 300, 600, and 1,200 ppm H₂-silica, and only 1,200 ppm H₂-silica caused a 2.4-fold increase in apoptosis in HEEpiCs compared with the control group as the index of Bax/Bcl-2. These findings come from a laboratory model and suggest molecular hydrogen's biological potential. Further research is needed to determine clinical relevance.
What the Researchers Tested
These findings should provide insights into the mechanism of inhibition of H₂-silica on human cancer cells in vitro.
How Molecular Hydrogen Was Used
The source material available to H2HUBB did not provide enough detail to identify the molecular hydrogen administration method.
Laboratory Model
These findings should provide insights into the mechanism of inhibition of H₂-silica on human cancer cells in vitro.
What Molecular Hydrogen Changed
KYSE-70 cells and HEEpiCs were generally inhibited by H₂-silica administration, and there was a significant proliferation-inhibitory effect in an H₂-silica concentration-dependent manner compared with the control group (P < 0.05) in KYSE-70. Apoptosis-inducing effect on KYSE-70 cells was observed in 10, 300, 600, and 1,200 ppm H₂-silica, and only 1,200 ppm H₂-silica caused a 2.4-fold increase in apoptosis in HEEpiCs compared with the control group as the index of Bax/Bcl-2.
H₂ Mechanisms / Biological Findings
Molecular hydrogen has antioxidant and antiapoptotic effects and a preventive effect on oxidative stress-induced cell death.
Authors’ Conclusion
A distinct authors’ conclusion was not available in the source material reviewed by H2HUBB.
Limitations Reported by the Authors
In the present study, the researchers investigated solidified hydrogen-occluding-silica (H₂-silica) that can release molecular hydrogen into cell culture medium because the use of hydrogen gas has strict handling limitations in hospital and medical facilities and laboratories, owing to its physicochemical characteristics.