Linking Alzheimer’s Disease and Type 2 Diabetes: Characterization and Inhibition of Cytotoxic Aβ and IAPP Hetero-Aggregates.
Kenana Al Adem, Aya Shanti, Amit Srivastava, Dirar Homouz, Sneha Ann Thomas, Mostafa Khair, Cesare Stefanini, Vincent Chan, Tae-Yeon Kim, Sungmun Lee · Frontiers in molecular biosciences · 2022
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In a laboratory model, showed that EGCG reduced hetero-aggregate formation and resulted in lower β-sheets content and higher unordered structures in IAPP-Aβ40-EGCG samples. The authors concluded that increasing evidence, particularly the co-deposition of Aβ and IAPP in both brain and pancreatic tissues, suggests that Aβ and IAPP cross-interaction may be responsible for a pathological link between AD and T2D. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen was evaluated in this publication and summarizes the source-grounded findings below. showed that EGCG reduced hetero-aggregate formation and resulted in lower β-sheets content and higher unordered structures in IAPP-Aβ40-EGCG samples.
What the Researchers Studied
The study used a in vitro cell-culture laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
HFIP is a well-known fluorinated alcohol that is highly polar and has strong hydrogen binding properties that induces the monomeric and un-aggregated conformations of the peptides. Hence, the peptides were kept in HFIP until the beginning of the experiment to ensure the removal of pre-existing aggregates and to maintain the peptides in monomeric conformations ( Kayed et al., 1999; Krampert et al., 2000; Stine et al., 2003; Yan et al., 2006; Yan et al., 2007; Andreetto et al., 2010; Yan et al., 2014 ). The co-aggregation profiles of equimolar mixture of IAPP:Aβ40 were acquired at (B) 10 µM:10 µM and (C) 20 µM:20 µM. (D) ThT fluorescence intensities of the freshly prepared samples (i.e., 0-Hour) of IAPP alone, Aβ40 alone and IAPP-Aβ40 at all examined concentrations. (E) Time evolution of inter-peptide hydrogen bonds formed between IAPP and Aβ40 in their hetero-dimer system. showed that EGCG reduced hetero-aggregate formation and resulted in lower β-sheets content and higher unordered structures in IAPP-Aβ40-EGCG samples. The authors concluded that increasing evidence, particularly the co-deposition of Aβ and IAPP in both brain and pancreatic tissues, suggests that Aβ and IAPP cross-interaction may be responsible for a pathological link between AD and T2D.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The study used a in vitro cell-culture laboratory experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.