Hydrogen (H2) Alleviates Osteoarthritis by Inhibiting Apoptosis and Inflammation via the JNK Signaling Pathway.
Hongwei Lu, Wei Wang, Xiaodiao Kang, Zeng Lin, Jun Pan, Shaowen Cheng, Jingdong Zhang · Journal of inflammation research · 2021
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Independent study record
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H2HUBB TAKEAWAY
In mice, the immunofluorescence of cleaved caspase-3 also indicated that H₂ suppressed the expression of cleaved caspase-3 in TBHP-induced chondrocytes ( Figure 5H ). These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas affected the outcomes measured in mice. The immunofluorescence of cleaved caspase-3 also indicated that H₂ suppressed the expression of cleaved caspase-3 in TBHP-induced chondrocytes ( Figure 5H ).
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 75% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 75% source-reported H₂ gas concentration; reported treatment duration: 1 min. The immunofluorescence of cleaved caspase-3 also indicated that H₂ suppressed the expression of cleaved caspase-3 in TBHP-induced chondrocytes ( Figure 5H ). The authors concluded that uncovered that hydrogen (H₂) could alleviate apoptosis response and ECM degradation in human chondrocytes via inhibiting the activation of the JNK signaling pathway. Hydrogen can inhibit inflammatory factors (ADAMTS5 and MMP13) and apoptosis factors (cleaved caspase-3, cytochrome c, and Bax) in TBHP-induced chondrocytes. hydrogen can suppress the activation of JNK signaling pathway, whereas the effect of hydrogen can be abolished by anisomycin (a JNK activator).
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The reported sample size was 6. The study used a preclinical animal experiment. The hydrogen delivery method was inhaled hydrogen gas. The source-reported hydrogen concentration was 75% source-reported H₂ gas concentration. The reported treatment duration was 1 min.
Limitations and Safety
Reported limitations: DISCUSSION: Osteoarthritis (OA) is a chronic joint degradation disease that is characterized by long-term pain and joint limitation.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.