Electrochemically Reduced Water Delays Mammary Tumors Growth in Mice and Inhibits Breast Cancer Cells Survival In Vitro.
Giovanni Vanni Frajese, Monica Benvenuto, Rosanna Mattera, Saverio Giampaoli, Elena Ambrosin, Roberta Bernardini, Maria Gabriella Giganti, Loredana Albonici, Ivan Dus, Vittorio Manzari, Andrea Modesti, Maurizio Mattei, Roberto Bei · Evidence-based complementary and alternative medicine : eCAM · 2018
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
ERW treatment induced apoptosis of breast cancer cell lines independently of the status of p53 and ER and PR receptors, electrochemical reduced water (ERW) has been proposed to have beneficial effects on human health due to its rich content of H₂ and the presence of platinum nanoparticles with antioxidant effects. The authors concluded that these results suggest a potential beneficial role of ERW in inhibiting cancer cells growth. These findings from a laboratory model add evidence about molecular hydrogen's biological effects in the model studied.
What the Findings Mean
H2HUBB reviewed how hydrogen-rich water affected the outcomes measured in In addition, ERW treatment induced apoptosis of breast cancer cell lines independently of the status of p53 and ER and PR receptors. Electrochemical reduced water (ERW) has been proposed to have beneficial effects on human health due to its rich content of H₂ and the presence of platinum nanoparticles with antioxidant effects.
What the Researchers Studied
The researchers studied In addition, ERW treatment induced apoptosis of breast cancer cell lines independently of the status of p53 and ER and PR receptors. The study used a in vitro cell-culture laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
The ERW produced by the generator has low ORP (-772 ± 51 mV), elevated concentration of dissolved hydrogen (H₂, 1327 ± 158), and an alkaline pH (10.8). During the experiments, cells were cultured with DMEM, high glucose (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) prepared by dilution of 10x DMEM with ERW, designated as ERW treatment (ERW: ORP -450 ± 37 mV, pH 7.3 ± 0.1, H₂ = 751 ± 136), or cultured with DMEM prepared by dilution of 10x DMEM with autoclaved ERW, designated as autoclaved treatment (autoclaved ERW: ORP +50 ± 6 mV, pH 7.3 ± 0.1, H₂ = 0). The researchers used DMEM liquid (Aurogene, Rome, Italy) only as a positive control medium to monitor the cell growth, because breast cancer cells were routinely maintained in this medium (CTR: ORP +50 ± 8 mV, pH 7.3 ± 0.1, H₂ = 0). Electrochemical reduced water (ERW) has been proposed to have beneficial effects on human health due to its rich content of H₂ and the presence of platinum nanoparticles with antioxidant effects. The authors concluded that these results suggest a potential beneficial role of ERW in inhibiting cancer cells growth. Previous in vivo studies with ERW focused on other factors: nephroprotective effect of ERW against cisplatin-induced kidney toxicity and oxidative damage in mice [ 42 ] and antioxidant and anti-inflammatory effects of hydrogen-rich water in alleviating ethanol-induced fatty liver in mice [ 67 ].
Why These Findings Matter
This publication adds useful evidence about where molecular hydrogen did and did not change the measured outcomes, helping define the larger therapeutic evidence base.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was In addition, ERW treatment induced apoptosis of breast cancer cell lines independently of the status of p53 and ER and PR receptors. The study used a in vitro cell-culture laboratory experiment. The hydrogen delivery method was hydrogen-rich water.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.