Hydrogen-rich medium protects human skin fibroblasts from high glucose or mannitol induced oxidative damage.
Pan Yu, Zhenxiang Wang, Xuejun Sun, Xiaohua Chen, Suyun Zeng, Liang Chen, Shirong Li · Biochemical and biophysical research communications · 2011
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
Interestingly, the use of a hydrogen-rich medium significantly reduced the level of intracellular O(2)(-), stabilized the ΔΨ and attenuated production of MDA, 8-OHdG and 3-NT which efficiently enhanced the antioxidative defense system and protected the HSFs from subsequent oxidative stress damage. This source-grounded publication contributes evidence relevant to molecular hydrogen and is retained in a transparent general research category when a more specific study design is not supported by the indexed record.
What the Publication Examined
Hydrogen-rich medium protects human skin fibroblasts from high glucose or mannitol induced oxidative damage.
How Molecular Hydrogen Was Addressed
A new antioxidant, hydrogen, can selectively neutralize hydroxyl radicals (()OH) and peroxynitrite (ONOO(-)) in cell-free systems, whereas it seldom reacts with other ROS. In the present study, the researchers investigated the protective effects of hydrogen-rich medium on human skin fibroblasts (HSFs) under oxidative stress. Interestingly, the use of a hydrogen-rich medium significantly reduced the level of intracellular O(2)(-), stabilized the ΔΨ and attenuated production of MDA, 8-OHdG and 3-NT which efficiently enhanced the antioxidative defense system and protected the HSFs from subsequent oxidative stress damage.
What the Publication Reported
Interestingly, the use of a hydrogen-rich medium significantly reduced the level of intracellular O(2)(-), stabilized the ΔΨ and attenuated production of MDA, 8-OHdG and 3-NT which efficiently enhanced the antioxidative defense system and protected the HSFs from subsequent oxidative stress damage.
H₂ Mechanisms / Biological Findings
Interestingly, the use of a hydrogen-rich medium significantly reduced the level of intracellular O(2)(-), stabilized the ΔΨ and attenuated production of MDA, 8-OHdG and 3-NT which efficiently enhanced the antioxidative defense system and protected the HSFs from subsequent oxidative stress damage. In other words, hydrogen decreased the excessive generation of intracellular O(2)(-) and elevated the cellular antioxidative defense. In the present study, the researchers investigated the protective effects of hydrogen-rich medium on human skin fibroblasts (HSFs) under oxidative stress.
Authors’ Conclusion
The authors concluded that based on the results, hydrogen may have applications in the treatment of skin diseases caused by diabetes.