Hydrogen Research Study
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Preclinical animal evidenceAnimal studyinhaled hydrogen gas

Molecular hydrogen exerts neuroprotection against cerebral ischemia-reperfusion injury via Nrf2/HO-1-dependent ferroptosis inhibition in mice

Wang Z, Feng W, Zhou J, Huang L, Chen H, Xue L · 2026

Research-use notice

Independent study record

Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.

H2HUBB Research Library branded molecular hydrogen research image
Primary topic Stroke and Brain Injury
Evidence type Preclinical animal evidence
Publication type Animal study
Hydrogen method inhaled hydrogen gas

H2HUBB TAKEAWAY

In SH‑SY5Y cells, H₂ improved cell viability and MMP, and reduced apoptosis and ferroptosis following OGD/R. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.

What the Findings Mean

H2HUBB reviewed how inhaled hydrogen gas affected the outcomes measured in C57BL/6 mice. In SH‑SY5Y cells, H₂ improved cell viability and MMP, and reduced apoptosis and ferroptosis following OGD/R.

What the Researchers Studied

The researchers studied C57BL/6 mice. The study used a preclinical animal experiment.

What Effects Did Molecular Hydrogen Have?

In SH‑SY5Y cells, H₂ improved cell viability and MMP, and reduced apoptosis and ferroptosis following OGD/R. The authors concluded that this study is the first to demonstrate that H₂ protects against CIRI by attenuating oxidative stress and ferroptosis via the Nrf2/GPX4/HO‑1 pathway, revealing a novel mechanism underlying hydrogen‑mediated neuroprotection. Molecular hydrogen(H₂)is a highly permeable, biosafe and selective antioxidant, which has emerged as a promising redox-modulating agent in various oxidative stress-related disorders.

Why These Findings Matter

These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.

How Strong Is This Evidence?

This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.

Technical Study Details

H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was C57BL/6 mice. The study used a preclinical animal experiment. The hydrogen delivery method was inhaled hydrogen gas.

Limitations and Safety

No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.

Original Study and H2HUBB Research Context

H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base. The original scholarly source is available at https://www.researchsquare.com/article/rs-9579320/v1.