Potent anti-inflammatory responses: Role of hydrogen in IL-1α dominated early phase systemic inflammation.
Youzhen Wei, Kun Wang, Yafang Zhang, Yi Duan, Yan Tian, Hongling Yin, Xuelian Fu, Zuan Ma, Jianjun Zhou, Min Yu, Qingbin Ni, Wenjie Tang · Frontiers in pharmacology · 2023
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Independent study record
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H2HUBB TAKEAWAY
In Inflammation in male C57/BL6J mice or RAW264.7 cells was induced with LPS, for which hydrogen was immediately administered until samples were taken, hydrogen alleviated LPS-induced inflammatory infiltration in the lung tissues of mice. These findings come from a laboratory model and suggest molecular hydrogen's biological potential. Further research is needed to determine clinical relevance.
What the Researchers Tested
Inflammation in male C57/BL6J mice or RAW264.7 cells was induced with LPS, for which hydrogen was immediately administered until samples were taken.
Laboratory Model
Inflammation in male C57/BL6J mice or RAW264.7 cells was induced with LPS, for which hydrogen was immediately administered until samples were taken.
How Molecular Hydrogen Was Used
Inflammation in male C57/BL6J mice or RAW264.7 cells was induced with LPS, for which hydrogen was immediately administered until samples were taken.
What Molecular Hydrogen Changed
Among the 23 inflammatory factors screened, LPS-induced upregulation of IL-1α etc. was significantly inhibited by hydrogen within 1 hour. The mRNA expression of MCP-1, MIP-1α, G-CSF, and RANTES was inhibited obviously by hydrogen at 0.5 and 1 h in mouse peritoneal macrophages. Hydrogen significantly blocked LPS or H₂O₂-induced upregulation of HIF-1α, and IL-1α in 0.5 h in RAW264.7 cells. Hydrogen alleviated LPS-induced inflammatory infiltration in the lung tissues of mice.
H₂ Mechanisms / Biological Findings
Hydrogen alleviated LPS-induced inflammatory infiltration in the lung tissues of mice. Among the 23 inflammatory factors screened, LPS-induced upregulation of IL-1α etc. was significantly inhibited by hydrogen within 1 hour. The target of the inhibitive LPS-induced-inflammatory action of hydrogen is chemokines in macrophages in the peritoneal cavity. Introduction: It has been proven that hydrogen has obvious anti-inflammatory effects in animal experiments and clinical practice.
Authors’ Conclusion
The authors concluded that the results suggested that hydrogen is potentially inhibitive against inflammation by inhibiting HIF-1α and IL-1α release at early occurrence. The target of the inhibitive LPS-induced-inflammatory action of hydrogen is chemokines in macrophages in the peritoneal cavity. This study provides direct experimental evidence for quickly controlling inflammation with the translational application of a hydrogen-assisted protocol.