[Role of Nrf2 in the protective effects of hydrogen against cerebral dysfunction in septic mice].
Lingling Liu, Keliang Xie, Hongguang Chen, Xiaoqing Dong, Guolin Wang, Yonghao Yu · Zhonghua wei zhong bing ji jiu yi xue · 2014
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Independent study record
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H2HUBB TAKEAWAY
In mice, compared with the sham operation and hydrogen control groups, in the sepsis group, the number of normal pyramidal neurons in the hippocampal CA1 region was markedly reduced, the apoptotic index was marked increased, the expressions of nucleus and total Nrf2 were partly increased, the activities of SOD and CAT in the hippocampus were significantly decreased. The levels of MDA and 8-iso-PGF2α were markedly increased, the escape latency at day 4 to 8 after operation was significantly extended, and there was no difference in swimming speed, the percentage of time in the target quadrant and the times of the platform crossing were significantly decreased on probe day. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in mice. Compared with the sham operation and hydrogen control groups, in the sepsis group, the number of normal pyramidal neurons in the hippocampal CA1 region was markedly reduced, the apoptotic index was marked increased, the expressions of nucleus and total Nrf2 were partly increased, the activities of SOD and CAT in the hippocampus were significantly decreased. The levels of MDA and 8-iso-PGF2α were markedly increased, the escape latency at day 4 to 8 after operation was significantly extended, and there was no difference in swimming speed, the percentage of time in the target quadrant and the times of the platform crossing were significantly decreased on probe day.
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
2% inhaled H₂ concentration; reported treatment duration: 1 hour. Compared with the sham operation and hydrogen control groups, in the sepsis group, the number of normal pyramidal neurons in the hippocampal CA1 region was markedly reduced, the apoptotic index was marked increased, the expressions of nucleus and total Nrf2 were partly increased, the activities of SOD and CAT in the hippocampus were significantly decreased. The levels of MDA and 8-iso-PGF2α were markedly increased, the escape latency at day 4 to 8 after operation was significantly extended, and there was no difference in swimming speed, the percentage of time in the target quadrant and the times of the platform crossing were significantly decreased on probe day. The authors concluded that hydrogen inhalation can ameliorate pathological injury in brain and impairment of learning and memory abilities of septic mice, which may be associated with the up-regulation of Nrf2, the increase of antioxidant enzymes activities and the decrease of oxidative products.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The study used a preclinical animal experiment. The reported hydrogen concentration was 2% inhaled H₂ concentration. The reported treatment duration was 1 hour.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.