Inhalation of hydrogen gas ameliorates glyoxylate-induced calcium oxalate deposition and renal oxidative stress in mice.
Zhongjiang Peng, Wei Chen, Li Wang, Zhouheng Ye, Songyan Gao, Xuejun Sun, Zhiyong Guo · International journal of clinical and experimental pathology · 2015
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In mice, the renal expressions of osteopontin (OPN), CD44, monocyte chemoattractant protein-1 (MCP-1) and interleukin-10 (IL-10) were markedly increased in glyoxylate-treated mice, and H₂ significantly attenuated the increase of OPN, CD44 and MCP-1 but upregulated the expression of IL-10. These results are preclinical and suggest molecular hydrogen's therapeutic potential in the condition studied. Further human research is needed to establish clinical effectiveness.
What the Researchers Studied
The study examined mice.
How Molecular Hydrogen Was Used
Two days before administration of glyoxylate, inhalation of H₂ for 30 min per day was initiated and continued for 7 days. According to levels of urine calcium excretion, renal calcium deposition, a serum excretion of kidney injury molecule-1 (KIM-1) assay and a TUNEL assay, inhalation of H₂ could successfully decrease the CaOx crystallizations and protect against renal injury. The findings demonstrate that inhalation of H₂ reduces renal crystallization, renal oxidative injury and inflammation and it may be a candidate agent with few adverse effects for prevention of nephrolithiasis.
What the Researchers Found
According to levels of urine calcium excretion, renal calcium deposition, a serum excretion of kidney injury molecule-1 (KIM-1) assay and a TUNEL assay, inhalation of H₂ could successfully decrease the CaOx crystallizations and protect against renal injury. The renal expressions of osteopontin (OPN), CD44, monocyte chemoattractant protein-1 (MCP-1) and interleukin-10 (IL-10) were markedly increased in glyoxylate-treated mice, and H₂ significantly attenuated the increase of OPN, CD44 and MCP-1 but upregulated the expression of IL-10.
H₂ Mechanisms / Biological Findings
The aim of this study is to evaluate the protective effect and underlying mechanism of hydrogen gas (H₂) to glyoxylate induced renal calcium oxalate (CaOx) crystal deposition in mice.
Authors’ Conclusion
The authors concluded that crystal deposition in the kidneys is associated with oxidative stress, which was indicated by increased levels of renal malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHdG) and decreased activities of superoxide dismutase (SOD), glutathione (GSH) and catalase (CAT).