Phosphorylation-mediated PI3K-Art signalling pathway as a therapeutic mechanism in the hydrogen-induced alleviation of brain injury in septic mice.
Yuanyuan Bai, Li Li, Beibei Dong, Wanjie Ma, Hongguang Chen, Yonghao Yu · Journal of cellular and molecular medicine · 2022
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In mice, 5 In the present study, the researchers quantitatively assessed the changes in protein phosphorylation in the H₂ ‐treated CLP model mice and identified functions and signalling cascades associated with the phosphoproteins that exhibited significant changes in abundance. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas affected the outcomes measured in mice. 5 In the present study, the researchers quantitatively assessed the changes in protein phosphorylation in the H₂ ‐treated CLP model mice and identified functions and signalling cascades associated with the phosphoproteins that exhibited significant changes in abundance.
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 2% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 2% source-reported H₂ gas concentration; reported treatment duration: 60 min. 5 In the present study, the researchers quantitatively assessed the changes in protein phosphorylation in the H₂ ‐treated CLP model mice and identified functions and signalling cascades associated with the phosphoproteins that exhibited significant changes in abundance. FIGURE 6 Differentially expressed phosphoproteins (DPPs) (Rps6, Pten, Ywhag, mTOR, Magi1, and Pkn2) participate in the PI3K‐AKT signalling pathway were significantly different in the therapeutic mechanism of hydrogen in CLP‐induced mice. (A) The western blot were used to identify the differentially expressed phosphoproteins and the total proteins.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The study used a preclinical animal experiment. The hydrogen delivery method was inhaled hydrogen gas. The source-reported hydrogen concentration was 2% source-reported H₂ gas concentration. The reported treatment duration was 60 min.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.