Protective effects of hydrogen enriched saline on liver ischemia reperfusion injury by reducing oxidative stress and HMGB1 release.
Yantao Liu, Liqun Yang, Kunming Tao, Marcela P Vizcaychipi, Dafydd M Lloyd, Xuejun Sun, Michael G Irwin, Daqing Ma, Weifeng Yu · BMC gastroenterology · 2014
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Independent study record
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H2HUBB TAKEAWAY
In rats, 10 ml/kg hydrogen-enriched saline treatment significantly reduced ALT to 284.1 ± 34.0 IU/l after 2 hours of reperfusion ( p < 0.05). In contrast, histological evidence of tissue damage following hydrogen-enriched saline treatment was greatly reduced and cell necrosis was not easily detected (Figure 2 D) at 2 hours post reperfusion. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in rats. 10 ml/kg hydrogen-enriched saline treatment significantly reduced ALT to 284.1 ± 34.0 IU/l after 2 hours of reperfusion ( p < 0.05).
What the Researchers Studied
The researchers studied rats. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Male Sprague Dawley rats were subject to partial warm liver ischemia/reperfusion injury with intraperitoneal injection of either normal saline or hydrogen enriched saline at dose of 2.5, 5 or 10 ml/kg 10 minutes before reperfusion. 10 ml/kg hydrogen-enriched saline treatment significantly reduced ALT to 284.1 ± 34.0 IU/l after 2 hours of reperfusion ( p < 0.05). The authors concluded that these results suggest that, in the authors' model, hydrogen enriched saline treatment is protective against liver ischemia-reperfusion injury. Hydrogen enriched saline treatment inhibited HMGB1 expression and release, reflecting a reduced local and systemic inflammatory response to hepatic ischemia reperfusion. Hydrogen treatment was recently associated with down-regulation of the expression of HMGB1 and pro-inflammatory cytokines during sepsis and myocardial IRI, but it is not known whether hydrogen has an effect on HMGB1 in liver IRI.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was rats. The study used a preclinical animal experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.