Hydrogen Research Study
← Back to Research Library
Preclinical animal evidenceAnimal studyinhaled hydrogen gas

Safety of inhaled hydrogen gas in healthy mice.

Alexis R Cole, Ali Raza, Humera Ahmed, Brian D Polizzotti, Robert F Padera, Nick Andrews, John N Kheir · Medical gas research · 2019

Research-use notice

Independent study record

Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.

H2HUBB Research Library branded molecular hydrogen research image
Primary topic Safety, Dosage, and Pharmacokinetics
Evidence type Preclinical animal evidence
Publication type Animal study
Hydrogen method inhaled hydrogen gas

H2HUBB TAKEAWAY

In mice, similarly, there was no significant change in the total SHIRPA score, although hydrogen-treated mice exhibited significantly lower spontaneous locomotor activity (P 0.05 for all). These preclinical findings add evidence about the molecular-hydrogen effects, outcomes, and biological pathways measured in this model.

What the Findings Mean

H2HUBB reviewed how inhaled hydrogen gas affected the outcomes measured in mice. Similarly, there was no significant change in the total SHIRPA score, although hydrogen-treated mice exhibited significantly lower spontaneous locomotor activity (P 0.05 for all).

What the Researchers Studied

The researchers studied mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.

What Effects Did Molecular Hydrogen Have?

Source-reported hydrogen concentration: 3.2% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 3.2% source-reported H₂ gas concentration; reported treatment duration: 72 hours. Similarly, there was no significant change in the total SHIRPA score, although hydrogen-treated mice exhibited significantly lower spontaneous locomotor activity (P 0.05 for all). The authors concluded that hydrogen gas does not appear to cause significant adverse effects when administered to healthy mice for 72 hours, with the possible exception of decreased spontaneous locomotor activity.

Why These Findings Matter

This publication adds useful evidence about where molecular hydrogen did and did not change the measured outcomes, helping define the larger therapeutic evidence base.

How Strong Is This Evidence?

This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.

Technical Study Details

H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The study used a preclinical animal experiment. The hydrogen delivery method was inhaled hydrogen gas. The source-reported hydrogen concentration was 3.2% source-reported H₂ gas concentration. The reported treatment duration was 72 hours.

Limitations and Safety

Safety information: The purpose of this work was to determine the safety of inhaled hydrogen gas in healthy animals.

Original Study and H2HUBB Research Context

H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.