Hydrogen Research Study
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Preclinical animal evidenceAnimal studyhydrogen-rich water

富氢水抑制离心运动后骨骼肌线粒体氧化应激和炎症反应

Wang Lei · 《中国组织工程研究》杂志 · 2015

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Independent study record

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H2HUBB Research Library branded molecular hydrogen research image
Primary topic Molecular Hydrogen Overview
Evidence type Preclinical animal evidence
Publication type Animal study
Hydrogen method hydrogen-rich water

H2HUBB TAKEAWAY

In Sprague Dawley rats, the study examined whether hydrogen-rich water could protect skeletal muscle from eccentric-exercise injury and explored the underlying mechanisms. These results are preclinical and suggest molecular hydrogen's therapeutic potential in the condition studied. Further human research is needed to establish clinical effectiveness.

What the Researchers Studied

The study examined whether hydrogen-rich water could protect skeletal muscle from eccentric-exercise injury and explored the underlying mechanisms.

How Molecular Hydrogen Was Used

Rats in the eccentric exercise+hydrogen-rich water group were subjected to intraperitoneal injection of hydrogen-rich water (10 mL/kg) immediately after exercise; and rats in the eccentric exercise+saline group were administrated with normal saline after exercise.

What the Researchers Found

Rats in the eccentric exercise+hydrogen-rich water group were subjected to intraperitoneal injection of hydrogen-rich water (10 mL/kg) immediately after exercise; and rats in the eccentric exercise+saline group were administrated with normal saline after exercise. It is unclear whether hydrogen-rich water can be used to protect skeletal muscle injury induced by eccentric exercise, as well as the relative mechanism. Forty Sprague Dawley rats were randomly divided into four groups: control group, eccentric exercise group, eccentric exercise+saline group, and eccentric exercise+hydrogen-rich water group.

H₂ Mechanisms / Biological Findings

It is unclear whether hydrogen-rich water can be used to protect skeletal muscle injury induced by eccentric exercise, as well as the relative mechanism. To observe the effect of hydrogen-rich water on the mitochondrial oxidative stress and inflammation in rat skeletal muscle after eccentric exercise, and to investigate the relative signaling pathway of hydrogen-rich water. intervention after eccentric exercise could markedly enhance the mitochondrial Sirtuin-3 expression, improve the mitochondrial membrane potential and activity of manganese superoxide dismutase, down-regulate the mitochondrial reactive oxygen species generation and mitochondrial DNA oxidative damage, thus inhibiting inflammatory cytokines expression, such as NLRP3 and interleukin-1β. hydrogen-rich water could improve mitochondrial energy metabolism and antioxidant capacity through up-regulation of Sirtuin-3, which in turn inhibits eccentric exercise-induced mitochondrial oxidative stress and secondary inflammation in the skeletal muscle.

Authors’ Conclusion

The authors concluded that hydrogen-rich water could directly scavenge reactive oxygen species.