A link between gut community metabolism and pathogenesis: molecular hydrogen-stimulated glucarate catabolism aids Salmonella virulence.
Reena Lamichhane-Khadka, Stéphane L Benoit, Susan E Maier, Robert J Maier · Open biology · 2013
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In mice, addition of H₂ enhanced the growth of both WT and RLK6, though the total CFU ml −1 of the mutant strain remained much lower than that of the WT in both H₂ -added and H₂ -absent conditions. These preclinical findings add evidence about the molecular-hydrogen effects, outcomes, and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in mice. Addition of H₂ enhanced the growth of both WT and RLK6, though the total CFU ml −1 of the mutant strain remained much lower than that of the WT in both H₂ -added and H₂ -absent conditions.
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Anaerobic conditions with H₂ were established by sparging the 15 ml of culture medium with inflow and outflow needles in sealed 165 ml bottles with N₂ for 15 min, then with anaerobic mix (10% H₂, 5% CO₂ and 85% N₂ ) for 20 min. H₂ gas from a 100% cylinder was then injected to bring the volume of added H₂ to 20% partial pressure [ 21 ]. Addition of H₂ enhanced the growth of both WT and RLK6, though the total CFU ml −1 of the mutant strain remained much lower than that of the WT in both H₂ -added and H₂ -absent conditions. The authors concluded that the results link a gut microbiome-produced diffusible metabolite to augmenting bacterial pathogenesis. This H₂ -mediated increased gene expression is most likely related to hydrogenases generating a lower potential cellular redox state, such that gene-expression-related effectors are either directly or indirectly activated.
Why These Findings Matter
This publication adds useful evidence about where molecular hydrogen did and did not change the measured outcomes, helping define the larger therapeutic evidence base.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The reported sample size was 4. The study used a preclinical animal experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.