Constitutive hydrogen inhalation prevents vascular remodeling via reduction of oxidative stress.
Takeshi Kiyoi, Shuang Liu, Erika Takemasa, Hirotomo Nakaoka, Naohito Hato, Masaki Mogi · PloS one · 2020
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Independent study record
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H2HUBB TAKEAWAY
In C57BL/6 mice, although the increase in superoxide anion production did not significantly differ between the hydrogen and control groups, DNA damage was decreased as a result of reduction of reactive oxygen species such as hydroxyl radical (⋅OH) and peroxynitrite (ONOO-) in the hydrogen group. These results are preclinical and add to understanding of how molecular hydrogen affects the condition studied. Further human research is needed to determine its clinical relevance.
What the Researchers Studied
C57BL/6 mice
How Molecular Hydrogen Was Used
Molecular hydrogen is thought to have an inhibitory effect on oxidative stress, thereby attenuating the onset and progression of various diseases including cardiovascular disease; however, few reports have assessed the preventive effect of constitutive inhalation of hydrogen gas on of vascular remodeling. Here, the researchers investigated the effect of constitutive inhalation of hydrogen gas on vascular neointima formation using a cuff-induced vascular injury mouse model. After constitutive inhalation of compressed hydrogen gas (O₂ 21%, N₂ 77.7%, hydrogen 1.3%) or compressed air only (O₂ 21%, N₂ 79%) by C57BL/6 mice for 2 weeks from 8 weeks of age in a closed chamber, inflammatory cuff injury was induced by polyethylene cuff placement around the femoral artery under anesthesia, and hydrogen gas administration was continued until sampling of the femoral artery.
What the Researchers Found
Although the increase in superoxide anion production did not significantly differ between the hydrogen and control groups, DNA damage was decreased as a result of reduction of reactive oxygen species such as hydroxyl radical (⋅OH) and peroxynitrite (ONOO-) in the hydrogen group.
Biological or Mechanistic Findings
These results demonstrate that constitutive inhalation of hydrogen gas attenuates vascular remodeling partly via reduction of oxidative stress, suggesting that constitutive inhalation of hydrogen gas at a safe concentration in the living environment could be an effective strategy for prevention of vascular diseases such as atherosclerosis. Molecular hydrogen is thought to have an inhibitory effect on oxidative stress, thereby attenuating the onset and progression of various diseases including cardiovascular disease; however, few reports have assessed the preventive effect of constitutive inhalation of hydrogen gas on of vascular remodeling. After constitutive inhalation of compressed hydrogen gas (O₂ 21%, N₂ 77.7%, hydrogen 1.3%) or compressed air only (O₂ 21%, N₂ 79%) by C57BL/6 mice for 2 weeks from 8 weeks of age in a closed chamber, inflammatory cuff injury was induced by polyethylene cuff placement around the femoral artery under anesthesia, and hydrogen gas administration was continued until sampling of the femoral artery.
Authors’ Conclusion
The authors concluded that these results demonstrate that constitutive inhalation of hydrogen gas attenuates vascular remodeling partly via reduction of oxidative stress, suggesting that constitutive inhalation of hydrogen gas at a safe concentration in the living environment could be an effective strategy for prevention of vascular diseases such as atherosclerosis. These results are preclinical and add to understanding of how molecular hydrogen affects the condition studied. Further human research is needed to determine its clinical relevance.