Coral Hydrate, a Novel Antioxidant, Improves Alcohol Intoxication in Mice.
Hung-Tsung Wu, Ting-Hsing Chao, Horng-Yih Ou, Liang-Miin Tsai · Antioxidants (Basel, Switzerland) · 2022
Research-use notice
Independent study record
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H2HUBB TAKEAWAY
The researchers found that the redox potential of hydrogenated water changed drastically within 1 h, whereas coral hydrate exhibited much better stability, sustaining its redox potential for at least 24 h ( Figure 1 ). These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in mice. The researchers found that the redox potential of hydrogenated water changed drastically within 1 h, whereas coral hydrate exhibited much better stability, sustaining its redox potential for at least 24 h ( Figure 1 ).
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment.
What Effects Did Molecular Hydrogen Have?
Porous coral materials with or without hydrogenation were purchased from HoHo Biotech Co., Ltd. (Taipei, Taiwan). To generate hydrogenated water, hydrogen gas (99.9%, Yun-Shan, Tainan, Taiwan) was bubbled directly into Milli-Q water at 1 L/min for 10 min. The hydrogen content was 1200 ppb, pH 7.15, and Eh −556 mV. The researchers found that the redox potential of hydrogenated water changed drastically within 1 h, whereas coral hydrate exhibited much better stability, sustaining its redox potential for at least 24 h ( Figure 1 ). The authors concluded that taken together, the data suggest that coral hydrate might be an effective novel treatment for alcohol intoxication. The membranes were blocked with 10% skim milk in Tris buffered-saline with 0.05% Tween 20 for 1 h at room temperature and then incubated with 1:1000 primary antibody against catalase (Cell Signaling, Danvers, MA, USA), manganese superoxide dismutase (MnSOD; Cell Signaling), glutathione peroxidase (GPx; Cell Signaling), alcohol dehydrogenase (ADH; Abcam) acetaldehyde dehydrogenase (ALDH; Abcam), or actin (Millipore, Temecula, CA, USA) at 4 °C overnight.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The reported sample size was 6. The study used a preclinical animal experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.