Suppressive effects of electrochemically reduced water on matrix metalloproteinase-2 activities and in vitro invasion of human fibrosarcoma HT1080 cells.
Tomoya Kinjo, Jun Ye, Hanxu Yan, Takeki Hamasaki, Hidekazu Nakanishi, Kazuko Toh, Noboru Nakamichi, Shigeru Kabayama, Kiichiro Teruya, Sanetaka Shirahata · Cytotechnology · 2012
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In It has been demonstrated that hydrogen peroxide (H(2)O(2)) is directly associated with elevated matrix metalloproteinase-2 (MMP-2) expression in several cell lines, electrochemically reduced water (ERW), produced near the cathode during electrolysis, and scavenges intracellular H(2)O(2) in human fibrosarcoma HT1080 cells. These findings come from a laboratory model and suggest molecular hydrogen's biological potential. Further research is needed to determine clinical relevance. This electrolyzed-reduced-water cellular study is included under H2HUBB's early hydrogen-water policy. The original intervention should be reported as electrochemically reduced water, without claiming a measured H₂ dose unless the source reports one.
What the Researchers Tested
It has been demonstrated that hydrogen peroxide (H(2)O(2)) is directly associated with elevated matrix metalloproteinase-2 (MMP-2) expression in several cell lines.
Laboratory Model
It has been demonstrated that hydrogen peroxide (H(2)O(2)) is directly associated with elevated matrix metalloproteinase-2 (MMP-2) expression in several cell lines.
How Molecular Hydrogen Was Used
It has been demonstrated that hydrogen peroxide (H(2)O(2)) is directly associated with elevated matrix metalloproteinase-2 (MMP-2) expression in several cell lines.
What Molecular Hydrogen Changed
Electrochemically reduced water (ERW), produced near the cathode during electrolysis, and scavenges intracellular H(2)O(2) in human fibrosarcoma HT1080 cells. RT-PCR and zymography analyses revealed that when HT1080 cells were treated with ERW, the gene expression of MMP-2 and membrane type 1 MMP and activation of MMP-2 was repressed, resulting in decreased invasion of the cells into matrigel.
H₂ Mechanisms / Biological Findings
The source material reviewed did not establish a specific molecular hydrogen mechanism for the reported findings.
Authors’ Conclusion
The authors concluded that these results indicate that the inhibitory effect of ERW on tumor invasion is due to, at least in part, its antioxidative effect.
H2HUBB Evidence Boundary
This electrolyzed-reduced-water cellular study is included under H2HUBB's early hydrogen-water policy. The original intervention should be reported as electrochemically reduced water, without claiming a measured H₂ dose unless the source reports one.