Hydrogen gas therapy: A promising approach for sepsis management post-burn injury by modulating inflammation, oxidative stress, and wound healing.
Pan Yu, Nan Hong, Genwang Wang, Shuqiang Chen, Zhipeng Zhao · CytoJournal · 2025
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In vitro assays showed that burn serum reduced fibroblast proliferation and increased apoptosis (P < 0.01), which hydrogen gas mitigated by rescuing cell viability and reducing apoptosis (P < 0.01). These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in rats. In vitro assays showed that burn serum reduced fibroblast proliferation and increased apoptosis (P < 0.01), which hydrogen gas mitigated by rescuing cell viability and reducing apoptosis (P < 0.01).
What the Researchers Studied
The researchers studied rats. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Then, the RSF cells were treated with fetal bovine serum (FBS) (SH30406.03, Hyclone, Logan, Utah, USA), FBS, and hydrogen gas (generated using a hydrogen generator, AYANHC500ML, AnYan Instrument, Hangzhou, China), burned rat serum, and hydrogen gas, normal rat serum, and hydrogen gas separately. The stained cells were then analyzed using a flow cytometer (FACSVerse, Becton, Dickinson and Company, Franklin Lakes, NJ, USA). The data were processed using FlowJo (v10.6.2, Becton, Dickinson and Company, Franklin Lakes, NJ, USA). [ 29, 30 ] RSF cells were plated at 3 × 10^4 cells/mL in a 48-well plate, cultured for 24 h, and then treated with FBS (SH30406.03, Hyclone, Logan, Utah, USA), FBS and hydrogen gas (generated using a hydrogen generator, AYAN-HC500ML, AnYan Instrument, Hangzhou, China), burned rat serum, and hydrogen gas, normal rat serum, and hydrogen gas separately for another 48 h. In vitro assays showed that burn serum reduced fibroblast proliferation and increased apoptosis (P < 0.01), which hydrogen gas mitigated by rescuing cell viability and reducing apoptosis (P < 0.01). Enzyme-linked immunosorbent assay revealed burn serum-induced increases in the levels of inflammatory cytokines and oxidative stress markers, with decreases in antioxidant enzymes (P < 0.01), which hydrogen gas reversed (P < 0.05). WB analysis suggested hydrogen gas's anti-inflammatory and proliferative effects by modulating signaling pathways (P < 0.01).
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was rats. The reported sample size was 7. The study used a preclinical animal experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.