Hydrogen gas with hypothermic machine perfusion induces lipidomic alterations in donation-after-cardiac-death rat livers.
Yusuke Minami, Siddabasave Gowda B B Gowda, Kengo Shibata, Sodai Sakamoto, Divyavani Gowda, Tsuyoshi Shimamura, Akinobu Taketomi, Hitoshi Chiba, Moto Fukai, Shu-Ping Hui · Journal of lipid research · 2026
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In rats, compared with MP, MP-H₂ treatment reduced lysophosphatidylinositol (LPI) levels and the LPI/phosphatidylinositol ratio while increasing phosphatidic acid (PA) species such as PA (18:0/18:1) and PA (18:0/20:4). These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas affected the outcomes measured in rats. Compared with MP, MP-H₂ treatment reduced lysophosphatidylinositol (LPI) levels and the LPI/phosphatidylinositol ratio while increasing phosphatidic acid (PA) species such as PA (18:0/18:1) and PA (18:0/20:4).
What the Researchers Studied
The researchers studied rats. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 4% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 4% source-reported H₂ gas concentration. Compared with MP, MP-H₂ treatment reduced lysophosphatidylinositol (LPI) levels and the LPI/phosphatidylinositol ratio while increasing phosphatidic acid (PA) species such as PA (18:0/18:1) and PA (18:0/20:4). The authors concluded that the restoration of key lipid species associated with mitochondrial integrity and membrane remodeling suggests that hydrogen gas supports bioenergetic recovery and limits oxidative membrane damage during reperfusion. Further investigation is required to determine how the combined application of MP and hydrogen gas influences specific metabolic pathways of sphingolipids.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was rats. The study used a preclinical animal experiment. The hydrogen delivery method was inhaled hydrogen gas. The source-reported hydrogen concentration was 4% source-reported H₂ gas concentration.
Limitations and Safety
Reported limitations: A principal limitation of this study is the lack of identification of key lipid-metabolizing enzymes, such as those involved in the Lands’ cycle and diacylglycerol kinase, which precludes a direct mechanistic interpretation of the lipidomic alterations.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.