Hydrogen-Generating Silica Material Prevents UVA-ray-Induced Cellular Oxidative Stress, Cell Death, Collagen Loss and Melanogenesis in Human Cells and 3D Skin Equivalents.
Li Xiao, Mai Mochizuki, Taka Nakahara, Nobuhiko Miwa · Antioxidants (Basel, Switzerland) · 2021
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Independent study record
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H2HUBB TAKEAWAY
In Ultraviolet-A (UVA) irradiation induces harmful effects on skin cells and accelerates skin aging through oxidative stress, uVA irradiation greatly decreased the cell viability to 30.3% of that in sham-irradiated HGFs (NC) at 48 h post-UVA irradiation ( Figure 4 B). The authors concluded that in conclusion, the data suggested that ULH-002 could protect human keratinocytes and fibroblasts from UVA-induced injuries, prevent the loss of type IV and I collagens, as well as reduce melanogenesis. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in Ultraviolet-A (UVA) irradiation induces harmful effects on skin cells and accelerates skin aging through oxidative stress. UVA irradiation greatly decreased the cell viability to 30.3% of that in sham-irradiated HGFs (NC) at 48 h post-UVA irradiation ( Figure 4 B).
What the Researchers Studied
The researchers studied Ultraviolet-A (UVA) irradiation induces harmful effects on skin cells and accelerates skin aging through oxidative stress. The study used a in vitro cell-culture laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Reported treatment duration: 5 min. UVA irradiation greatly decreased the cell viability to 30.3% of that in sham-irradiated HGFs (NC) at 48 h post-UVA irradiation ( Figure 4 B). The authors concluded that in conclusion, the data suggested that ULH-002 could protect human keratinocytes and fibroblasts from UVA-induced injuries, prevent the loss of type IV and I collagens, as well as reduce melanogenesis. As shown in Figure 2, ULH-002 solutions (0.1–0.3 mg/mL) significantly reduced both UVA-induced cytoplasm and nuclei ROS in both HaCaT cells and HGFs, suggesting that ULH-002 can prevent oxidative stress in skin cells probably through the dual permeations into the cell membrane and nucleus membrane, during the elimination of hydrogen bubbles from the occluded silica micro-porous cavities.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was Ultraviolet-A (UVA) irradiation induces harmful effects on skin cells and accelerates skin aging through oxidative stress. The study used a in vitro cell-culture laboratory experiment. The reported treatment duration was 5 min.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.