Hydrogen improves the efficacy of tetrandrine in the treatment of silicosis by inhibiting vascular endothelial mesenchymal transition caused by oxidative stress.
Kang Xiao, Ming Li, Yingwen Mu, Yuxin Sun, Sirui Wang, Shangya Chen, Jiazi Ma, Mao Cao, Yong Yang, Hua Shao, Xuansheng Ding, Guanqun Cui, Zhongjun Du · Frontiers in bioengineering and biotechnology · 2025
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Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In To detect the content of Tet in the lung tissues of different groups of mice, the mice were divided into three groups include PBS control group, silica group and Tet group, combined inhalation of hydrogen (H₂) improved both the severity of silicosis and Tet concentration in lung tissue (from 50 ng/mg to 80 ng/mg). The authors concluded that hydrogen can improve the tissue concentration of tetrandrine by anti-OS-induced EndMT. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas affected the outcomes measured in To detect the content of Tet in the lung tissues of different groups of mice, the mice were divided into three groups include PBS control group, silica group and Tet group. Combined inhalation of hydrogen (H₂) improved both the severity of silicosis and Tet concentration in lung tissue (from 50 ng/mg to 80 ng/mg).
What the Researchers Studied
The researchers studied To detect the content of Tet in the lung tissues of different groups of mice, the mice were divided into three groups include PBS control group, silica group and Tet group. The study used a laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Reported treatment duration: 6 days. Combined inhalation of hydrogen (H₂) improved both the severity of silicosis and Tet concentration in lung tissue (from 50 ng/mg to 80 ng/mg). The authors concluded that hydrogen can improve the tissue concentration of tetrandrine by anti-OS-induced EndMT. This mechanism could explain the observed increase in tissue concentrations of tetrandrine following hydrogen administration. The membranes were incubated with the primary antibodies, namely, Pink1 (1:1000 dilution, Proteintech, United States, 23274-1-AP), LC3 (1:1000 dilution, Cell Signaling Technology, United States, 12741T), sequestosome 1 (SQSTM1/p62) (1:1000 dilution, Cell Signaling Technology, United States, 5114T), Parkin (1:2000 dilution, Proteintech, United States, 14060-1-AP), Kelch-like ECH-associated protein 1 (Keap1) (1:1000 dilution, Proteintech, United States, 60027-1-Ig), nuclear factor E2-related factor 2 (Nrf2) (1:2000 dilution, Proteintech, United States, 16396-1-AP), B- cell lymphoma – 2 (Bcl-2) (1:2000 dilution, Proteintech, United States, 26593-1-AP), Bcl – 2-associated X protein (Bax) (1:5000 dilution, Proteintech, United States, 50599-2-Ig), Cytochrome C (Cyto C) (1:5000, Proteintech, United States, 10993-1-AP), Caspase 3 (1:1000 dilution, Proteintech, United States, 19677-1-AP) and Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:500000 dilution, Proteintech, United States, 60004-1-Ig).
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was To detect the content of Tet in the lung tissues of different groups of mice, the mice were divided into three groups include PBS control group, silica group and Tet group. The study used a laboratory experiment. The hydrogen delivery method was inhaled hydrogen gas. The reported treatment duration was 6 days.
Limitations and Safety
Reported limitations: Firstly, the researchers did not perform the power calculation, the small sample size may restrict the statistical significance and universality of the results. Safety information: Regarding the safety of H₂ use, although in most of the existing studies, no severe adverse reactions directly caused by H₂ have been reported, H₂ is a natural product of anaerobic bacteria metabolism in the intestines.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.