Hydrogen inhalation ameliorates oxidative stress in transplantation induced intestinal graft injury.
B M Buchholz, D J Kaczorowski, R Sugimoto, R Yang, Y Wang, T R Billiar, K R McCurry, A J Bauer, A Nakao · American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2008
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Independent study record
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H2HUBB TAKEAWAY
In rats, histopathological mucosal erosion and increased gut permeability indicated a breakdown in posttransplant mucosal barrier function which was significantly attenuated by hydrogen treatment. These results are preclinical and suggest molecular hydrogen's therapeutic potential in the condition studied. Further human research is needed to establish clinical effectiveness.
What the Researchers Studied
The study examined rats.
How Molecular Hydrogen Was Used
Both donor and recipient received perioperative air or 2% hydrogen inhalation. Administration of perioperative hydrogen gas may be a potent and clinically applicable therapeutic strategy for intestinal I/R injury.
What the Researchers Found
Hydrogen treatment resulted in significantly improved gastrointestinal transit, as well as jejunal smooth muscle contractility in response to bethanechol. The transplant induced upregulation in the inflammatory mediators CCL2, IL-1 beta, IL-6 and TNF-alpha were mitigated by hydrogen. Hydrogen significantly diminished lipid peroxidation compared to elevated tissue malondialdehyde levels in air-treated grafts demonstrating an antioxidant effect. Histopathological mucosal erosion and increased gut permeability indicated a breakdown in posttransplant mucosal barrier function which was significantly attenuated by hydrogen treatment.
H₂ Mechanisms / Biological Findings
The transplant induced upregulation in the inflammatory mediators CCL2, IL-1 beta, IL-6 and TNF-alpha were mitigated by hydrogen. In recipient lung, hydrogen treatment also resulted in a significant abatement in inflammatory mRNA induction and reduced neutrophil recruitment.
Authors’ Conclusion
The authors concluded that histopathological mucosal erosion and increased gut permeability indicated a breakdown in posttransplant mucosal barrier function which was significantly attenuated by hydrogen treatment.