Hydrogen Inhalation Attenuates Oxidative Stress Related Endothelial Cells Injury After Subarachnoid Hemorrhage in Rats.
Kai Zhuang, Yu-Chun Zuo, Prativa Sherchan, Ji-Kai Wang, Xiao-Xin Yan, Fei Liu · Frontiers in neuroscience · 2019
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In a laboratory model, immunohistochemistry staining for fibrinogen showed significantly increased microthrombi counts in both cortex and hippocampus after SAH which was reduced with H₂ inhalation three times for 120 min each in the SAH + H₂ group compared to SAH group ( p < 0.05; Figures 5A,B ). The authors concluded that hydrogen inhalation can ameliorate oxidative stress related endothelial cells injury in the brain and improve neurobehavioral outcomes in rats following SAH. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas was evaluated in this publication and summarizes the source-grounded findings below. Immunohistochemistry staining for fibrinogen showed significantly increased microthrombi counts in both cortex and hippocampus after SAH which was reduced with H₂ inhalation three times for 120 min each in the SAH + H₂ group compared to SAH group ( p < 0.05; Figures 5A,B ).
What the Researchers Studied
The study used a laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 3.3% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 3.3% source-reported H₂ gas concentration; reported treatment duration: 3 days. Immunohistochemistry staining for fibrinogen showed significantly increased microthrombi counts in both cortex and hippocampus after SAH which was reduced with H₂ inhalation three times for 120 min each in the SAH + H₂ group compared to SAH group ( p < 0.05; Figures 5A,B ). The authors concluded that hydrogen inhalation can ameliorate oxidative stress related endothelial cells injury in the brain and improve neurobehavioral outcomes in rats following SAH. Hydrogen inhalation reduced the expression of these inflammatory and apoptotic makers in the vessels, brain edema, microthrombi formation, and vasospasm in rats with SAH relative to control.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The reported sample size was 2. The study used a laboratory experiment. The hydrogen delivery method was inhaled hydrogen gas. The source-reported hydrogen concentration was 3.3% source-reported H₂ gas concentration. The reported treatment duration was 3 days.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.