Pharmacological postconditioning with lactic acid and hydrogen rich saline alleviates myocardial reperfusion injury in rats.
Guoming Zhang, Song Gao, Xiaoyan Li, Lulu Zhang, Hong Tan, Lin Xu, Yaoyu Chen, Yongjian Geng, Yanliang Lin, Benjamin Aertker, Yuanyuan Sun · Scientific reports · 2015
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In rats, these data suggested that pharmacological postconditioning with lactic acid and hydrogen rich saline nearly replicates the benefits of mechanical postconditioning. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in rats. These data suggested that pharmacological postconditioning with lactic acid and hydrogen rich saline nearly replicates the benefits of mechanical postconditioning.
What the Researchers Studied
The researchers studied rats. The study used a preclinical animal experiment.
What Effects Did Molecular Hydrogen Have?
According to the randomized and controlled principle, rats were divided into the 6 groups: (1) animals in the sham group underwent thoracotomy and NS injection (60 µL), without any other ischemia treatment. (2) the reperfusion-injury group (R/I) received NS (60 µL) and routine continuous reperfusion treatment; (3) the mechanical postconditioning group (M-Post) received NS (60 µL) and postconditioning treatment which consisted of 4 × 20 s cycles of alternating reperfusion/re-occlusion, and initiated immediately upon the onset of reperfusion; (4) the lactic acid group (Lac) received lactic acid (60 µL) and routine ischemia-reperfusion treatment; (5) the hydrogen rich saline group (Hyd) received hydrogen rich saline (60 µL) and routine ischemia-reperfusion treatment; (6) the lactic acid and hydrogen rich saline group (Lac + Hyd) received a combination of lactic acid (60 µL) and hydrogen rich saline (60 µL) followed by routine ischemia-reperfusion treatment. These data suggested that pharmacological postconditioning with lactic acid and hydrogen rich saline nearly replicates the benefits of mechanical postconditioning. Although similar effects on apoptotic cell death, ROS generation, tissue pH and infarct size were observed, exploration of the MAPK pathway revealed that there may be differences in the upstream effects of mechanical postconditioning and hydrogen rich saline with lactic acid.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was rats. The study used a preclinical animal experiment.
Limitations and Safety
Reported limitations: A second limitation was the use of MDA and SOD activity in lieu of directly measuring ROS generation.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.