Hydrogen Metabolism in Helicobacter pylori Plays a Role in Gastric Carcinogenesis through Facilitating CagA Translocation.
Ge Wang, Judith Romero-Gallo, Stéphane L Benoit, M Blanca Piazuelo, Ricardo L Dominguez, Douglas R Morgan, Richard M Peek, Robert J Maier · mBio · 2016
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In rabbits, by examining the hydrogenase activities from well-defined clinical H. pylori isolates, the researchers observed that strains isolated from cancer patients (n = 6) have a significantly higher hydrogenase (H₂/O₂) activity than the strains isolated from gastritis patients (n = 6), further supporting an association between H. pylori hydrogenase activity and gastric carcinogenesis in humans. These preclinical findings add evidence about the molecular-hydrogen effects, outcomes, and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in rabbit. By examining the hydrogenase activities from well-defined clinical H. pylori isolates, the researchers observed that strains isolated from cancer patients (n = 6) have a significantly higher hydrogenase (H₂/O₂) activity than the strains isolated from gastritis patients (n = 6), further supporting an association between H. pylori hydrogenase activity and gastric carcinogenesis in humans.
What the Researchers Studied
The researchers studied rabbit. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Cells were grown in the presence of H₂ (in sealed anaerobic jars with CampyPak Plus microaerophilic envelopes; Becton, Dickinson, Sparks, MD), harvested and resuspended in phosphate-buffered saline (PBS), cell density (optical density at 600 nm [OD 600 ]) was measured, and hydrogen uptake was monitored using a previously described amperometric method ( 16, 17 ). Hydrogenase activity was expressed as nanomoles of H₂ used per minute per 10 9 cells. By examining the hydrogenase activities from well-defined clinical H. pylori isolates, the researchers observed that strains isolated from cancer patients (n = 6) have a significantly higher hydrogenase (H₂/O₂) activity than the strains isolated from gastritis patients (n = 6), further supporting an association between H. pylori hydrogenase activity and gastric carcinogenesis in humans. The authors concluded that the Δhyd strain showed a decreased frequency of DNA transformation, suggesting that H. pylori hydrogenase is also involved in energizing the DNA uptake apparatus. On binding and then “splitting” of hydrogen gas by membrane-associated nickel-containing hydrogenases, the energy contained in the low-potential electrons is conserved by a combination of transmembrane potential and proton gradient coupling mechanisms ( 34 ).
Why These Findings Matter
This publication adds useful evidence about where molecular hydrogen did and did not change the measured outcomes, helping define the larger therapeutic evidence base.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was rabbit. The study used a preclinical animal experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.