Hydrogen protects auditory hair cells from cisplatin-induced free radicals.
Yayoi S Kikkawa, Takayuki Nakagawa, Mirei Taniguchi, Juichi Ito · Neuroscience letters · 2014
Research-use notice
Independent study record
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H2HUBB TAKEAWAY
In Following 48-h incubation, the presence of intact auditory hair cells was assayed by phalloidin staining, hydroxyphenyl fluorescein (HPF) staining of the spiral ganglion showed that formation of hydroxyl radicals was successfully reduced in hydrogen-treated cochleae. The authors concluded that these data suggest that molecular hydrogen can protect auditory tissues against cisplatin toxicity, thus providing an additional strategy to protect against drug-induced inner ear damage. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in Following 48-h incubation, the presence of intact auditory hair cells was assayed by phalloidin staining. Hydroxyphenyl fluorescein (HPF) staining of the spiral ganglion showed that formation of hydroxyl radicals was successfully reduced in hydrogen-treated cochleae.
What the Researchers Studied
The researchers studied Following 48-h incubation, the presence of intact auditory hair cells was assayed by phalloidin staining. The study used a in vitro cell-culture laboratory experiment.
What Effects Did Molecular Hydrogen Have?
Molecular hydrogen was recently established as an antioxidant that selectively reduces ROS, and has been reported to protect the central nervous system, liver, kidney and cochlea from oxidative stress. The purpose of this study was to evaluate the potential of molecular hydrogen to protect cochleae against cisplatin. Hydroxyphenyl fluorescein (HPF) staining of the spiral ganglion showed that formation of hydroxyl radicals was successfully reduced in hydrogen-treated cochleae. The authors concluded that these data suggest that molecular hydrogen can protect auditory tissues against cisplatin toxicity, thus providing an additional strategy to protect against drug-induced inner ear damage.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was Following 48-h incubation, the presence of intact auditory hair cells was assayed by phalloidin staining. The study used a in vitro cell-culture laboratory experiment.
Limitations and Safety
Reported limitations: However, its maximum dose is often limited by severe ototoxicity.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.