Hydrogen regulates mitochondrial quality to protect glial cells and alleviates sepsis-associated encephalopathy by Nrf2/YY1 complex promoting HO-1 expression.
Yang Zhang, Juntai Chen, Haidong Wu, Lixin Li, Xuejia Yang, Keguan Lai, Jingyu Bao, Keliang Xie, Yonghao Yu · International immunopharmacology · 2023
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Independent study record
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H2HUBB TAKEAWAY
In Animal models of SAE were generated by cecal ligation and puncture, and the SAE model was established by in vitro LPS stimulation, finally, hydrogen treatment improved the results of behavioral detection, apoptosis, Nrf2, HO-1, PGC-1α, TFAM, OPA1, DRP1, MFN2, PARKIN, PINK1, and cytokines in SAE in vivo. The authors concluded that hydrogen improved cell injury and mitochondrial quality, which were associated with HO-1 expression promoted by the Nrf2/YY1 complex in vitro. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in Animal models of SAE were generated by cecal ligation and puncture, and the SAE model was established by in vitro LPS stimulation. Finally, hydrogen treatment improved the results of behavioral detection, apoptosis, Nrf2, HO-1, PGC-1α, TFAM, OPA1, DRP1, MFN2, PARKIN, PINK1, and cytokines in SAE in vivo.
What the Researchers Studied
The researchers studied Animal models of SAE were generated by cecal ligation and puncture, and the SAE model was established by in vitro LPS stimulation. The study used a in vitro cell-culture laboratory experiment.
What Effects Did Molecular Hydrogen Have?
MTT, lactate dehydrogenase (LDH), reactive oxygen species (ROS), heme oxygenase-1 (HO-1) activity, mitochondrial membrane potential (MMP), and cellular apoptosis assays were used to determine the effect of hydrogen on astrocytes and microglia stimulated by LPSs. The effects of hydrogen treatment in the SAE mouse model were investigated using Morris water maze and Y-maze analyses. Finally, hydrogen treatment improved the results of behavioral detection, apoptosis, Nrf2, HO-1, PGC-1α, TFAM, OPA1, DRP1, MFN2, PARKIN, PINK1, and cytokines in SAE in vivo. The authors concluded that hydrogen improved cell injury and mitochondrial quality, which were associated with HO-1 expression promoted by the Nrf2/YY1 complex in vitro. Hydrogen plays a protective role in different diseases; however, the detailed mechanism of hydrogen-treated disease remains unclear. The purpose of this study was to investigate the effect of hydrogen on SAE in vitro and in vivo and the mechanism of hydrogen in mitochondrial dynamics and its function in astrocytes and microglia stimulated by lipopolysaccharides (LPSs).
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was Animal models of SAE were generated by cecal ligation and puncture, and the SAE model was established by in vitro LPS stimulation. The study used a in vitro cell-culture laboratory experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.