Hydrogen-rich saline alleviates cardiomyocyte apoptosis by reducing expression of calpain1 via miR-124-3p.
Xiaofei Xue, Wang Xi, Wei Li, Jian Xiao, Zhinong Wang, Yufeng Zhang · ESC heart failure · 2023
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H2HUBB TAKEAWAY
In mice, it was also found that compared with the hydrogen-rich saline group, overexpression of calpain1 increased caspase-3 activities, promoted cleaved-caspase3 and Bax protein expressions, and correspondingly decreased Bcl-2, further reducing cell viability. These results are preclinical and suggest molecular hydrogen's therapeutic potential in the condition studied. Further human research is needed to establish clinical effectiveness.
What the Researchers Studied
The study examined mETHODS AND.
How Molecular Hydrogen Was Used
Molecular hydrogen was administered as molecular hydrogen has been exhibited a protective function in heart diseases. the authors' previous study demonstrated that hydrogen-rich saline could scavenge free radicals selectively and alleviate the inflammatory response in the myocardial ischaemia/reperfusion (i/r) injury, but the underlying mechanism has not been fully clarified.
What the Researchers Found
In this study, the researchers found that miR-124-3p was significantly decreased in both I/R and H/R models, while it was partially ameliorated by hydrogen-rich saline pretreatment. hydrogen-rich saline treatment also alleviated ischaemia-induced apoptotic cell death and increased cell viability during I/R process, whereas silencing expression of miR-124-3p abolished this protective effect. It was also found that compared with the hydrogen-rich saline group, overexpression of calpain1 increased caspase-3 activities, promoted cleaved-caspase3 and Bax protein expressions, and correspondingly decreased Bcl-2, further reducing cell viability.
H₂ Mechanisms / Biological Findings
The authors' previous study demonstrated that hydrogen-rich saline could scavenge free radicals selectively and alleviate the inflammatory response in the myocardial ischaemia/reperfusion (I/R) injury, but the underlying mechanism has not been fully clarified.
Authors’ Conclusion
The authors concluded that the present study showed a protective effect of hydrogen-rich saline on I/R injury, which may be associated with miR-124-3p-calpain1 signalling pathway.