Hydrogen-rich saline ameliorated LPS-induced acute lung injury via autophagy inhibition through the ROS/AMPK/mTOR pathway in mice.
Yong Wang, Jinghua Zhang, Jinsong Bo, Xuefen Wang, Jingnan Zhu · Experimental biology and medicine (Maywood, N.J.) · 2019
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In mice, evidence is growing that hydrogen-rich saline may be an effective drug for the prevention and treatment of ALI. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in mice. Evidence is growing that hydrogen-rich saline may be an effective drug for the prevention and treatment of ALI.
What the Researchers Studied
The researchers studied mice. The study used a preclinical animal experiment.
What Effects Did Molecular Hydrogen Have?
Evidence is growing that hydrogen-rich saline may be an effective drug for the prevention and treatment of ALI. The authors concluded that in conclusion, the results indicated that hydrogen-rich saline ameliorat- ed LPS-induced ALI by inhibiting autophagy over- activation via the ROS/AMPK/mTOR pathway. Pretreatment with hydrogen-rich saline markedly inhibited the expression of Beclin-1 and the LC3II/I ratio; ameliorated lung histopathological changes, malondialdehyde content and wet/dry ratio; and reduced protein content and infiltration of inflammatory cells in bronchoalveolar lavage fluid. Original Research Hydrogen-rich saline ameliorated LPS-induced acute lung injury via autophagy inhibition through the ROS/AMPK/mTOR pathway in mice Yong Wang 1, Jinghua Zhang 2, Jinsong Bo 1, Xuefen Wang 1 and Jingnan Zhu 1 1Department of Clinical Laboratory, Zhejiang Shaoxing Shangyu People’s Hospital, Shaoxing 312353, P.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was mice. The study used a preclinical animal experiment.
Limitations and Safety
Safety information: 11,12 H₂ is the lightest chemical element and can easily diffuse through membranes into cytosol and organelles, exerting anti-oxidation, anti-inflammation, and anti-apoptotic effects.13,14 Under physiological conditions, breathing low concentrations of H₂ has been proven to be relatively safe. But as it has explosive property, the use of H₂ is limited in clinical practice.15 In studies, H₂ is often dissolved in a sol- vent like physiological saline (hydrogen-rich saline) under high pressure to observe its effects in the treatment and prevention of diseases.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base. The original scholarly source is available at https://www.ebm-journal.org/journals/experimental-biology-and-medicine/articles/10.1177/1535370219847941/pdf.