Hydrogen-Rich Saline Inhibits Lipopolysaccharide-Induced Acute Lung Injury and Endothelial Dysfunction by Regulating Autophagy through mTOR/TFEB Signaling Pathway.
Zhiling Fu, Ze Zhang, Xiuying Wu, Jin Zhang · BioMed research international · 2020
Research-use notice
Independent study record
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H2HUBB TAKEAWAY
In In vitro, HPMEC models of ALI were treated with LPS, iL-1 β, IL-6, and TNF- α were significantly decreased and IL-10 was significantly increased in the HRS group, compared with that in the ALI group ( P < 0.05) ( Figure 1(b) ). The authors concluded that the results confirmed the protection of HRS in LPS-induced ALI by regulating apoptosis through inhibiting the mTOR/TFEB signaling pathway. These findings from a laboratory model add evidence about molecular hydrogen's biological effects in the model studied.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in In vitro, HPMEC models of ALI were treated with LPS. IL-1 β, IL-6, and TNF- α were significantly decreased and IL-10 was significantly increased in the HRS group, compared with that in the ALI group ( P < 0.05) ( Figure 1(b) ).
What the Researchers Studied
The researchers studied In vitro, HPMEC models of ALI were treated with LPS. The study used a in vitro cell-culture laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Reported treatment duration: 1 hour. IL-1 β, IL-6, and TNF- α were significantly decreased and IL-10 was significantly increased in the HRS group, compared with that in the ALI group ( P < 0.05) ( Figure 1(b) ). The authors concluded that the results confirmed the protection of HRS in LPS-induced ALI by regulating apoptosis through inhibiting the mTOR/TFEB signaling pathway. The addition of saturated hydrogen saline inhibited the inflammatory response of ALI in rats, enhanced the autophagy level, upregulated LC3-II and Beclin-1, reduced P62, and promoted the degradation of autophagosomes of HPMEC cells.
Why These Findings Matter
This publication adds useful evidence about where molecular hydrogen did and did not change the measured outcomes, helping define the larger therapeutic evidence base.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was In vitro, HPMEC models of ALI were treated with LPS. The study used a in vitro cell-culture laboratory experiment. The reported treatment duration was 1 hour.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.