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Preclinical animal evidenceAnimal studyOther or not reported

Hydrogen-rich saline protects intestinal epithelial tight junction barrier in rats with intestinal ischemia-reperfusion injury by inhibiting endoplasmic reticulum stress-induced apoptosis pathway.

Shuai Jiang, Qizhong Fan, Ming Xu, Fengchun Cheng, Zhihui Li, Guojian Ding, Lei Geng, Tingliang Fu · Journal of pediatric surgery · 2020

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Research-use notice

Independent study record

Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.

H2HUBB Research Library branded molecular hydrogen research image
Primary topic Gastrointestinal Health
Evidence type Preclinical animal evidence
Publication type Animal study
Hydrogen method Other or not reported

H2HUBB TAKEAWAY

Compared with rats in the I/R group, the Chiu score of ileum damage in the hydrogen-rich saline group and 4-PBA group were lower. These results are preclinical and suggest molecular hydrogen's therapeutic potential in the condition studied. Further human research is needed to establish clinical effectiveness.

What the Researchers Studied

The study examined to investigate the effects of hydrogen-rich saline (HRS) on intestinal epithelial tight junction (TJ) barrier in rats with intestinal ischemia-reperfusion injury (IIRI).

How Molecular Hydrogen Was Used

The source material available to H2HUBB did not provide enough detail to identify the molecular hydrogen administration method.

What the Researchers Found

Increased expression of TJ proteins occludin and ZO-1 by reducing various parameters of ERS and ERS-induced apoptosis evidenced by down-regulation of the protein levels of GRP78, XBP1, CHOP and caspase-3 were shown in the HRS and 4-PBA groups.

H₂ Mechanisms / Biological Findings

Imunohistochemistry, immunofluorescence and Western blot were used to detect key proteins in intestinal epithelial TJs, ERS, and ERS-induced apoptosis, including occludin, zonula occludens-1 (ZO-1), glucose-regulated protein 78 (GRP78), X-box binding protein-1 (XBP1), C/EBP-homologous protein (CHOP) and caspase-3.

Authors’ Conclusion

This study suggested that inhibition of excessive ERS and ERS-induced apoptosis by HRS may reduce intestinal epithelial cells damage and maintain the integrity of intestinal epithelial TJ barrier in rats with IIRI.

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