Hydrogen saline offers neuroprotection by reducing oxidative stress in a focal cerebral ischemia-reperfusion rat model.
Ying Liu, Wenwu Liu, Xuejun Sun, Runping Li, Qiang Sun, Jianmei Cai, Zhimin Kang, Shijun Lv, John H Zhang, Wei Zhang · Medical gas research · 2011
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Independent study record
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H2HUBB TAKEAWAY
In rats, hydrogen saline significantly reduced the infarct volume and edema and improved the neurological function, when it was administered at 0, 3 and 6 h after reperfusion. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in rats. Hydrogen saline significantly reduced the infarct volume and edema and improved the neurological function, when it was administered at 0, 3 and 6 h after reperfusion.
What the Researchers Studied
The researchers studied rats. The study used a preclinical animal experiment.
What Effects Did Molecular Hydrogen Have?
However, rats in the MCAO plus hydrogen group received MCAO and intraperitoneal treatment with hydrogen saline (1 ml/100 g body weight) at designed time points (0, 3 or 6 h after reperfusion onset). The hydrogen was administered 0, 3 and 6 after reperfusion to explore the preferable therapeutic regimen and brains were removed as 24 h after reperfusion followed by detection of infarction and brain edema. Hydrogen saline significantly reduced the infarct ratio when applied at 0 or 3 h after reperfusion, but that was only slightly reduced when injected at 6 h after reperfusion (Figure 1A-B ). Hydrogen saline significantly reduced the infarct volume and edema and improved the neurological function, when it was administered at 0, 3 and 6 h after reperfusion. The authors concluded that the major findings were that hydrogen saline could reduce cerebral infarction and improve neurological function in the MCAO rat model, which were mediated by the reduction of oxidative stress (8-OHdG, nitrotyrosine and MDA) and inflammatory factors, and subsequent decrease of neuronal apoptosis (TUENL positive cells, expression of Bcl-2 and Bax, and caspase-3 activity). However, when compared with MCAO group, hydrogen saline applied 3 h after reperfusion significantly increased the e DISCUSSION. The findings were consistent with a recent study on hydrogen inhalation in which hydrogen inhalation also reduced the oxidative stress following ischemia/reperfusion [ 22 ].
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was rats. The reported sample size was 228. The study used a preclinical animal experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.