Ameliorating Role of Hydrogen-Rich Water Against NSAID-Induced Enteropathy via Reduction of ROS and Production of Short-Chain Fatty Acids.
Yoshihiro Akita, Masaaki Higashiyama, Chie Kurihara, Suguru Ito, Shin Nishii, Akinori Mizoguchi, Kenichi Inaba, Rina Tanemoto, Nao Sugihara, Yoshinori Hanawa, Akinori Wada, Kazuki Horiuchi, Yoshikiyo Okada, Kazuyuki Narimatsu, Shunsuke Komoto, Kengo Tomita, Fumie Takei, Yasushi Satoh, Masayuki Saruta, Ryota Hokari · Digestive diseases and sciences · 2023
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In Finally, cells from the macrophage cell line RAW264 were co-cultured with the supernatants of cecal contents, microscopic evaluation revealed that luminal ROS was significantly reduced and that HRW did not change the gut microbiota; however, FMT from HRW-treated animals ameliorated IND-induced enteropathy. The authors concluded that hydrogen-rich water ameliorated NSAID-induced enteropathy, not only via direct antioxidant effects but also via anti-inflammatory effects by increasing luminal SCFAs. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how hydrogen-rich water affected the outcomes measured in Finally, cells from the macrophage cell line RAW264 were co-cultured with the supernatants of cecal contents. Microscopic evaluation revealed that luminal ROS was significantly reduced and that HRW did not change the gut microbiota; however, FMT from HRW-treated animals ameliorated IND-induced enteropathy.
What the Researchers Studied
The researchers studied Finally, cells from the macrophage cell line RAW264 were co-cultured with the supernatants of cecal contents. The study used a in vitro cell-culture laboratory experiment.
What Effects Did Molecular Hydrogen Have?
Reported treatment duration: 5 days. Microscopic evaluation revealed that luminal ROS was significantly reduced and that HRW did not change the gut microbiota; however, FMT from HRW-treated animals ameliorated IND-induced enteropathy. The authors concluded that hydrogen-rich water ameliorated NSAID-induced enteropathy, not only via direct antioxidant effects but also via anti-inflammatory effects by increasing luminal SCFAs. Hydrogen-rich water significantly ameliorated IND-induced enteropathy histologically and reduced the expression of IND-induced inflammatory cytokines.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a in vitro cell-culture laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was Finally, cells from the macrophage cell line RAW264 were co-cultured with the supernatants of cecal contents. The study used a in vitro cell-culture laboratory experiment. The hydrogen delivery method was hydrogen-rich water. The reported treatment duration was 5 days.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.