Influence of Hydrogen-rich Saline on Hepatocyte Autophagy During Laparoscopic Liver Ischaemia-reperfusion Combined Resection Injury in Miniature Pigs.
Ge Bai, Hui Li, Yansong Ge, Qianzhen Zhang, Jiantao Zhang, Mingzi Chen, Tao Liu, Hongbin Wang · Journal of veterinary research · 2018
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Independent study record
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H2HUBB TAKEAWAY
In an animal model, however, ALT and AST activity significantly decreased at T3h and T1d and AKP activity significantly decreased at T1d in the IRI + hydrogen-rich saline group compared with the IRI group (P < 0.05, Figs 1A, 1B, and 1C). These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen was evaluated in this publication and summarizes the source-grounded findings below. However, ALT and AST activity significantly decreased at T3h and T1d and AKP activity significantly decreased at T1d in the IRI + hydrogen-rich saline group compared with the IRI group (P < 0.05, Figs 1A, 1B, and 1C).
What the Researchers Studied
The study used a preclinical animal experiment.
What Effects Did Molecular Hydrogen Have?
The pigs were randomly divided into three equal groups as follows: a sham operation group (sham group) in which pigs were subjected to laparoscopic surgery manipulating the liver lobes but without liver ischaemia-reperfusion or partial resection. A laparoscopic liver ischaemia-reperfusion combined resection injury group (IRI group), utilising a 4-portal approach where laparoscopic liver ischaemia and reperfusion as well as a partial resection were performed after a 60 min ischaemia; and a hydrogen-rich saline group (IRI + hydrogen-rich saline group) in which the laparoscopic operation was performed the same way as in the IRI group, but included portal vein catheterisation before reperfusion and administration of a portal vein injection of hydrogen-rich saline (10 mL/kg) 10 min prior to reperfusion. However, ALT and AST activity significantly decreased at T3h and T1d and AKP activity significantly decreased at T1d in the IRI + hydrogen-rich saline group compared with the IRI group (P < 0.05, Figs 1A, 1B, and 1C). The authors concluded that with hydrogen-rich saline could exert a protective effect against liver ischaemia-reperfusion combined resection injury through the reduction of oxidative stress and hepatocyte autophagy. As a scavenger of toxic ROS, hydrogen has a selective antioxidant effect, primarily scavenging hydroxyl radicals and nitrite anions, both of which exhibit potent oxidative toxicity.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The study used a preclinical animal experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.