Inhalation of high-concentration hydrogen gas attenuates cognitive deficits in a rat model of asphyxia induced-cardiac arrest.
Lei Huang, Richard L Applegate Ii, Patricia M Applegate, Lei Gong, Umut Ocak, Warren Boling, John H Zhang · Medical gas research · 2019
Research-use notice
Independent study record
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H2HUBB TAKEAWAY
In Neuronal degeneration within hippocampal Cornu Ammonis 1 (CA1) regions was examined by Fluoro-Jade staining ex vivo, both hydrogen gas treatment regimens significantly improved spatial learning function and attenuated neuronal degeneration within hippocampal CA1 regions at 18 days post-resuscitation. The authors concluded that water electrolysis derived 67% hydrogen gas may be an effective therapeutic approach for improving cognitive outcomes associated with global brain hypoxia-ischemia following cardiac arrest. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas affected the outcomes measured in Neuronal degeneration within hippocampal Cornu Ammonis 1 (CA1) regions was examined by Fluoro-Jade staining ex vivo. Both hydrogen gas treatment regimens significantly improved spatial learning function and attenuated neuronal degeneration within hippocampal CA1 regions at 18 days post-resuscitation.
What the Researchers Studied
The researchers studied Neuronal degeneration within hippocampal Cornu Ammonis 1 (CA1) regions was examined by Fluoro-Jade staining ex vivo. The study used a laboratory experiment.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 67% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 67% source-reported H₂ gas concentration; reported treatment duration: 1 hour. Both hydrogen gas treatment regimens significantly improved spatial learning function and attenuated neuronal degeneration within hippocampal CA1 regions at 18 days post-resuscitation. The authors concluded that water electrolysis derived 67% hydrogen gas may be an effective therapeutic approach for improving cognitive outcomes associated with global brain hypoxia-ischemia following cardiac arrest. Activation of the phosphoinositide 3-kinase/Akt pathway has been shown to play a role in the protective effects of 67% hydrogen gas in a rat model of liver ischemia-reperfusion. 28 Because of the importance phosphoinositide 3-kinase/Akt signaling in neuronal survival, 29 the researchers speculate that a similar mechanism of protection would underlie the hippocampal neuron protection provided by high concentration hydrogen.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The research population or model was Neuronal degeneration within hippocampal Cornu Ammonis 1 (CA1) regions was examined by Fluoro-Jade staining ex vivo. The reported sample size was 44. The study used a laboratory experiment. The hydrogen delivery method was inhaled hydrogen gas. The source-reported hydrogen concentration was 67% source-reported H₂ gas concentration. The reported treatment duration was 1 hour.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.