Inhaled hydrogen ameliorates endotoxin-induced bowel dysfunction.
Hiroyuki Sakata, Ayana Okamoto, Michiko Aoyama-Ishikawa, Hayato Yamashita, Keisuke Kohama, Noritomo Fujisaki, Taihei Yamada, Joji Kotani, Kohei Tsukahara, Atsuyoshi Iida, Atsunori Nakao · Acute medicine & surgery · 2017
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In vitro analysis of cytokine levels after LPS treatment of cultured macrophages showed an increase of interleukin-10 by hydrogen regardless of the presence of nitric oxide. The authors concluded that this study showed the protective effects of hydrogen inhalation on LPS-induced septic ileus through inhibition of inflammation in the muscularis propria. These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
What the Findings Mean
H2HUBB reviewed how inhaled hydrogen gas was evaluated in this publication and summarizes the source-grounded findings below. In vitro analysis of cytokine levels after LPS treatment of cultured macrophages showed an increase of interleukin-10 by hydrogen regardless of the presence of nitric oxide.
What the Researchers Studied
The study used a laboratory experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
Source-reported hydrogen concentration: 1.3% source-reported H₂ gas concentration; Source-reported hydrogen exposure: 1.3% source-reported H₂ gas concentration. In vitro analysis of cytokine levels after LPS treatment of cultured macrophages showed an increase of interleukin-10 by hydrogen regardless of the presence of nitric oxide. The authors concluded that this study showed the protective effects of hydrogen inhalation on LPS-induced septic ileus through inhibition of inflammation in the muscularis propria. Hydrogen significantly prevented LPS-induced bowel dysmotility and reduced leukocyte extravasation, as well as inhibition of inflammatory cytokine expression. Lipopolysaccharide challenge led to an increase of anti‐inflammatory IL‐10 hydrogen inhalation, which can decrease the early pro‐inflammatory cytokines in the intestinal muscularis propria.
Why These Findings Matter
These findings from a laboratory model add evidence supporting molecular hydrogen's biological and therapeutic potential in the model studied.
How Strong Is This Evidence?
This is laboratory evidence from a laboratory experiment. It is most informative for the biological mechanisms, cellular responses, or biochemical outcomes directly measured.
Technical Study Details
H2HUBB classifies this publication as laboratory study with laboratory or cellular evidence. The study used a laboratory experiment. The hydrogen delivery method was inhaled hydrogen gas. The source-reported hydrogen concentration was 1.3% source-reported H₂ gas concentration.
Limitations and Safety
No separate limitations or safety findings were identified in the current-study source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.