Therapeutic Effect of Hydrogen Injected Subcutaneously on Onion Poisoned Dogs.
Jinghua Zhao, Ming Zhang, Yue Li, Zhiheng Zhang, Mingzi Chen, Tao Liu, Jiantao Zhang, Anshan Shan · Journal of veterinary research · 2017
Research-use notice
Independent study record
Each H2HUBB study page organizes source-linked research details for educational use. Interpretation should remain proportional to the study design, population, controls, and limitations.
H2HUBB TAKEAWAY
In dogs, the capacity of medullary haematopoiesis that was based on reticulocyte counts, and the antioxidation in hydrogen group were higher compared with control group. These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
What the Findings Mean
H2HUBB reviewed how molecular hydrogen affected the outcomes measured in dogs. The capacity of medullary haematopoiesis that was based on reticulocyte counts, and the antioxidation in hydrogen group were higher compared with control group.
What the Researchers Studied
The researchers studied dogs. The study used a preclinical animal experiment. The comparison condition was control condition or baseline measurements.
What Effects Did Molecular Hydrogen Have?
All dogs were divided into two equal groups: control group (group C) in which the dogs did not undergo any treatment after poisoning, and hydrogen group (group H₂ ) in which the dogs were injected with hydrogen after poisoning. The dogs in group H₂ were injected hydrogen subcutaneously in the dose of 0.2 mL/kg ( 23 ) for 12 days, in which the Heinz bodies almost disappeared. Blood samples of two groups were collected from the jugular vein one week before feeding the onion powder (day –7), one day before injecting hydrogen (day 0), and 2 h after injection of hydrogen gas on days 1, 3, 5, 7, 9, and 12 of experimental treatment. The capacity of medullary haematopoiesis that was based on reticulocyte counts, and the antioxidation in hydrogen group were higher compared with control group. The authors concluded that accordingly, it was concluded that subcutaneous injection of hydrogen could alleviate the symptoms in onion poisoned dogs. The levels of leukocyte production, anaemia, red blood cell degeneration which was reflected by the values of Heinz body count, haemolytic ratio, and oxidative products in hydrogen treated group were lower than in control dogs on some days. The values of the haemolytic rate, which had positive correlation with the injury degree of erythrocyte membrane, were lower in group H₂ than in group C on some days, showing that hydrogen could inhibit the lipid peroxidation at a certain extent, reduce the oxidative damage of erythrocyte membrane, leading to reduction of the haemolysis degree in the poisoned dogs.
Why These Findings Matter
These preclinical findings add evidence supporting molecular hydrogen's therapeutic potential within the outcomes and biological pathways measured in this model.
How Strong Is This Evidence?
This is preclinical animal evidence from a preclinical animal experiment. It is most informative for the disease model, mechanisms, biomarkers, and outcomes directly measured in the study.
Technical Study Details
H2HUBB classifies this publication as animal study with preclinical animal evidence. The research population or model was dogs. The reported sample size was 16. The study used a preclinical animal experiment.
Limitations and Safety
No separate limitations or safety findings were identified in the source text available to H2HUBB.
Original Study and H2HUBB Research Context
H2HUBB presents this source-grounded research record as one contribution to the broader molecular-hydrogen evidence base.